TIMP-4 Antibody (MM0037-9F3) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB110-61003

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # MM0037-9F3

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all TIMP-4 Antibodies »

Product Specifications

Immunogen

Human recombinant TIMP-4

Specificity

No cross-reactivity to human TIMP-1, 2 and 3 is noted

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for TIMP-4 Antibody (MM0037-9F3) - Azide and BSA Free

Immunohistochemistry: TIMP-4 Antibody (MM0037-9F3) - Azide and BSA Free [NB110-61003]

Immunohistochemistry: TIMP-4 Antibody (MM0037-9F3) - Azide and BSA Free [NB110-61003]

Immunohistochemistry: TIMP-4 Antibody (MM0037-9F3) [NB110-61003] - Formalin fixed and paraffin embedded normal human placenta tissue using NB110-61003

Applications for TIMP-4 Antibody (MM0037-9F3) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:100-200

Immunohistochemistry-Paraffin

1:100-1:200

Western Blot

1:100-1:1000

Formulation, Preparation, and Storage

Purification

Protein G purified

Reconstitution

Reconstitute with sterilized PBS to a final concentration of 0.2 mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS. 0.025 mg size is provided in liquid form, PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: TIMP-4

The tissue inhibitors of metalloproteinases (TIMPs) are naturally occurring proteins that specifically inhibit matrix metalloproteinases and regulate extracellular matrix turnover and tissue remodeling by forming tight binding inhibitory complexes with the MMPs. Thus, TIMPs maintain the balance between matrix destruction and formation. An imbalance between MMPs and the associated TIMPs may play a significant role in the invasive phenotype of malignant tumors. The TIMP proteins share several structural features including six loops held in place by six disulfide bonds arranged in three knot-like structures. These proteins also contain twelve cysteine residues in conserved regions of the molecule that form six disulfide bonds, essential for the formation of native conformations, and the N terminal region that is necessary for inhibitory activities. The N terminus of each TIMP contains a consensus sequence (VIRAK) and each TIMP is translated with a 29 amino acid leader sequence that is cleaved off to produce the mature protein. The C terminal regions are divergent, which enhances the selectivity of inhibition and binding efficiency. Although the TIMP proteins share high homology, they may either be secreted extracellularly in soluble form (TIMP1, TIMP2 and TIMP4) or bind to extracellular matrix components (TIMP3). The MMPs and TIMPs can be divided into two groups with respect to gene expression: the majority exhibit inducible expression and a small number are produced constitutively or are expressed at very low levels and are not inducible. Among agents that induce MMP and TIMP production are the inflammatory cytokines TNF alpha and IL1 beta. A marked cell type specificity is a hallmark of both MMP and TIMP gene expression (i.e. a limited number of cell types can be induced to make these proteins). Tissue Inhibitor of Metalloproteinases 4 (TIMP4) was identified by molecular cloning. TIMP4 shows 37 % amino acid identity with TIMP1 and 51 % homology with TIMP2 and TIMP3. TIMP4 is secreted extracellularly, predominantly in heart and brain tissue. It may function in a tissue specific fashion in extracellular matrix (ECM) homeostasis. TIMP4 has a strong inhibitory effect on the invasion of human breast cancer cells across reconstituted basement membranes suggesting that TIMP4 may have an important role in inhibiting primary tumor growth and progression. The human TIMP4 gene has the chromosomal location of 3p25.

Long Name

Tissue Inhibitors of Metalloproteinases 4

Alternate Names

TIMP4

Entrez Gene IDs

7079 (Human)

Gene Symbol

TIMP4

Additional TIMP-4 Products

Product Documents for TIMP-4 Antibody (MM0037-9F3) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for TIMP-4 Antibody (MM0037-9F3) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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