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Tissue alpha-L-Fucosidase/FUCA1 Antibody (1D4)

Novus Biologicals, part of Bio-Techne | Catalog # H00002517-M01

Novus Biologicals, part of Bio-Techne
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H00002517-M01

Key Product Details

Species Reactivity

Human

Applications

Validated:

ELISA, Immunohistochemistry, Western Blot

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 1D4

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

FUCA1 (AAH17338, 23 a.a. ~ 461 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. VRRAQPPRRYTPDWPSLDSRPLPAWFDEAKFGVFIHWGVFSVPAWGSEWFWWHWQGEGRPQYQRFMRDNYPPGFSYADFGPQFTARFFHPEEWADLFQAAGAKYVVLTTKHHEGFTNWPSPVSWNWNSKDVGPHRDLVGELGTALRKRNIRYGLYHSLLEWFHPLYLLDKKNGFKTQHFVSAKTMPELYDLVNSYKPDLIWSDGEWECPDTYWNSTNFLSWLYNDSPVKDEVVVNDRWGQNCSCHHGGYYNCEDKFKPQSLPDHKWEMCTSIDKFSWGYRRDMALSDVTEESEIISELVQTVSLGGNYLLNIGPTKDGLIVPIFQERLLAVGKWLSINGEAIYASKPWRVQWEKNTTSVWYTSKGSAVYAIFLHWPENGVLNLESPITTSTTKITMLGIQGDLKWSTDPDKGLFISLPQLPPSAVPAEFAWTIKLTGVK

Specificity

FUCA1 - fucosidase, alpha-L- 1, tissue

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Tissue alpha-L-Fucosidase/FUCA1 Antibody (1D4)

Western Blot: Tissue alpha-L-Fucosidase/FUCA1 Antibody (1D4) [H00002517-M01]

Western Blot: Tissue alpha-L-Fucosidase/FUCA1 Antibody (1D4) [H00002517-M01]

Western Blot: Tissue alpha-L-Fucosidase/FUCA1 Antibody (1D4) [H00002517-M01] - Analysis of FUCA1 expression in transfected 293T cell line by FUCA1 monoclonal antibody (M01), clone 1D4. Lane 1: FUCA1 transfected lysate(53.2 KDa). Lane 2: Non-transfected lysate.
Western Blot: Tissue alpha-L-Fucosidase/FUCA1 Antibody (1D4) [H00002517-M01]

Western Blot: Tissue alpha-L-Fucosidase/FUCA1 Antibody (1D4) [H00002517-M01]

Western Blot: Tissue alpha-L-Fucosidase/FUCA1 Antibody (1D4) [H00002517-M01] - FUCA1 monoclonal antibody (M01), clone 1D4 Western Blot analysis of FUCA1 expression in K-562 ( Cat # L009V1 ).

Applications for Tissue alpha-L-Fucosidase/FUCA1 Antibody (1D4)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA. Use in Immunohistochemistry reported in scientific literature (PMID: 26204487).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Tissue alpha-L-Fucosidase/FUCA1

Fucosidosis is an autosomal recessive lysosomal storage disease caused by defective alpha-L-fucosidase with accumulation of fucose in the tissues. Different phenotypes include clinical features such as neurologic deterioration, growth retardation, visceromegaly, and seizures in a severe early form; coarse facial features, angiokeratoma corporis diffusum, spasticity and delayed psychomotor development in a longer surviving form; and an unusual spondylometaphyseoepiphyseal dysplasia in yet another form.[supplied by OMIM]

Alternate Names

FUCA1, Tissue alphaLFucosidase

Entrez Gene IDs

2517 (Human)

Gene Symbol

FUCA1

OMIM

230000 (Human)

Additional Tissue alpha-L-Fucosidase/FUCA1 Products

Product Documents for Tissue alpha-L-Fucosidase/FUCA1 Antibody (1D4)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Tissue alpha-L-Fucosidase/FUCA1 Antibody (1D4)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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