TLK1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-77982
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Chicken (93%), Mouse (96%), Rat (93%). Backed by our 100% Guarantee.
Applications
ELISA, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This affinity purified TLK1 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-Terminal region near amino acids 730-755 of human TLK1 (Tousled-like kinase) (isoform 1) protein. (Uniprot: Q9UKI8)
Reactivity Notes
A BLAST analysis was used to suggest reactivity with this protein from human, chimpanzee, orangutan, and dog sources based on 100% homology for the immunogen sequence. Cross reactivity with TLK1 protein from mouse, rat and chicken may occur as this sequence only varies by one amino acid residue (93% homology). Cross reactivity with TLK1 homologues from other sources has not been determined.
Specificity
This affinity purified antibody is directed against human TLK1 protein. This antibody reacts with both phosphorylated and non-phosphorylated forms of TLK1 at the S743 residue (isoform 1) of the protein. The antibody will react with all isoforms of TLK1. A BLAST analysis was used to suggest reactivity with this protein from human, chimpanzee, orangutan, and dog sources based on 100% homology for the immunogen sequence. Cross reactivity with TLK1 protein from mouse, rat and chicken may occur as this sequence only varies by one amino acid residue (93% homology). Cross reactivity with TLK1 homologues from other sources has not been determined.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This affinity purified antibody is directed against human Tlk-1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification
Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Scientific Data Images for TLK1 Antibody
![Western Blot: TLK1 Antibody [NBP1-77982]](https://resources.bio-techne.com/images/products/TLK1-Antibody-Western-Blot-NBP1-77982-img0002.jpg "Western Blot: TLK1 Antibody [NBP1-77982]")
Western Blot: TLK1 Antibody [NBP1-77982]
Western Blot: TLK1 Antibody [NBP1-77982] - Shows detection of a band ~64 kDa corresponding to isoform 3 of human Tlk-1 (arrowhead). Specific reactivity with this band is blocked when the antibody is pre-incubated with the immunizing peptide (data not shown). Approximately 30 ug of a HeLa nuclear extract was separated by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:250 for 2h at room temperature followed by washes and reaction with a 1:10,000 dilution of IRDye 800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature.
TLK1 Antibody
Western blot using Affinity Purified antibody shows detection of a band ~64 kDa corresponding to isoform 3 of human Tlk-1. Specific reactivity with this band is blocked when the antibody is pre-incubated with the immunizing peptide (data not shown). Approximately 30 ug of a HeLa nuclear extractApplications for TLK1 Antibody
Application
Recommended Usage
ELISA
1:10000-1:50000
Western Blot
1:200-1:2000
Application Notes
This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 86 kDa, 89 kDa or 64 kDa in size corresponding to isoforms of TLk-1 by western blotting in the appropriate cell lysate or extract. This antibody reacts with both phosphorylated and non-phosphorylated forms of Tlk-1 at the S743 residue (isoform 1) of the protein.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
Background: TLK1
Alternate Names
EC 2.7.11, EC 2.7.11.1, KIAA0137serine/threonine-protein kinase tousled-like 1, PKU-BETA, SNARE protein kinase SNAK, tousled-like kinase 1serine threonine protein kinase
Gene Symbol
TLK1
UniProt
Additional TLK1 Products
Product Documents for TLK1 Antibody
Product Specific Notices for TLK1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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