TLR1 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB100-56563
Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Mouse, Rat
Applications
Validated:
Dot Blot, Flow (Cell Surface), Flow (Intracellular), Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Cytometric Bead Assay Standard, Flow Cytometry, Flow Cytometry Control, IF/IHC, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
This antibody was developed against a KLH-conjugated synthetic peptide corresponding to a sequence within amino acids 400-450 of human TLR1.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for TLR1 Antibody - BSA Free
Flow Cytometry: TLR1 Antibody [NB100-56563]
Flow Cytometry: TLR1 Antibody [NB100-56563] - TLR1 expression pattern in primary BMNCs from 3 MM patients analyzed by flow cytometry. CD138-positive cells were gated from the total cell population. Staining for was compared with isotype-matched controls (NC). Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0060671) licensed under a CC-BY license.Western Blot: TLR1 Antibody [NB100-56563]
Western Blot: TLR1 Antibody [NB100-56563] - analysis of TLR1 in Ramos (A), Raw (B), and (C) TLR1 transfected cell lysate using this antibody. goat anti-rabbit HRP secondary antibody was used.Western Blot: TLR1 Antibody [NB100-56563]
Western Blot: TLR1 Antibody [NB100-56563] - All the cell lines expressed mRNA for TLR1 (Figure 1) and displayed a strong expression of TLR1 protein (Figure 2). Cell lysates were electrophoresed and blotted to PVDF membrane, which was probed with TLR1-specific antibody. To confirm the immunoreactivity of the antibody, different positive controls were included. Beta-actin was served as loading control and was used to normalize expression levels between cells. Data are representative for analysis of >=2 independent experiments. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0060671) licensed under a CC-BY license.Applications for TLR1 Antibody - BSA Free
Application
Recommended Usage
Flow (Cell Surface)
reported in scientific literature (Wong et al (2007); PMID 23593278)
Flow (Intracellular)
0.5-1 ug/1 million cells
Flow Cytometry
2-4 ug/ 1x10-6 cells
Immunohistochemistry
reported in scientific literature (PMID 27008696)
Immunohistochemistry-Paraffin
10 ug/ml
Western Blot
1-3 ug/ml
Application Notes
In Ramos, an 87 kDa band is observed. Flow (Intracellular) reported in scientific literature (PMID: 23593278)
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
BSA Free
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: TLR1
Long Name
Toll-like Receptor 1
Alternate Names
CD281
Gene Symbol
TLR1
UniProt
Additional TLR1 Products
Product Documents for TLR1 Antibody - BSA Free
Product Specific Notices for TLR1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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