Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

CyTOF-ready, Flow Cytometry, Western Blot

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2a Kappa Clone # 11G5

Format

Azide Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all TLR2 Antibodies »

Product Specifications

Immunogen

A portion of amino acids 400-450 of murine TLR2 was used as the immunogen for this antibody.

Reactivity Notes

The amino acid sequence used as immunogen is 80% in Rat, Chinese Hamster and Rabbit.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2a Kappa

Scientific Data Images for TLR2 Antibody (11G5) - Azide Free

Western Blot: TLR2 Antibody (11G5) [Azide Free] [NBP2-27166] - Analysis of TLR2 antibody. Mouse RAW cell lysate was probed using TLR2 antibody at 5 ug/ml. Goat anti-rat Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
Flow Cytometry: TLR2 Antibody (11G5) [Azide Free] [NBP2-27166] - Cell surface analysis using TLR2 antibody. Thyoglycollate-elicited peritoneal macrophages from C57BL/6 mice were tested with 2.5 ug of isotype control antibody (green) and 2.5 ug of TLR2 antibody (red). This antibody and an APC-conjugated secondary this antibody were used for this test.

Applications for TLR2 Antibody (11G5) - Azide Free

Application
Recommended Usage

Flow Cytometry

1ul/1 million cells

Western Blot

4-6ug/ml~
Application Notes
This monoclonal antibody only very weakly detects human TLR2 in Ramos cell lysate and is not recommended for human sample testing.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

0.2 ml PBS

Format

Azide Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TLR2

The Toll-like receptors (TLRs) in mammals comprise a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and an IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules (1). The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition (2). Ten human homologs of TLRs (TLR1-10) have been described (3). TLR2 is differentially expressed in human cells. TLR2 is expressed in tonsils, lymph nodes, and appendices, activated B-cells in germinal centers. CD14+ monocytes expressed the highest level of TLR2 followed by CD15+ granulocytes, and CD19+ B-cells, CD3+ T-cells, and CD56+ Nk cells did not express TLR2. The expression of TLR2 in different cell types is regulated by different immune response modifiers. For example, LPS, GM-CSF, IL-1, and IL-10 up regulates TLR2 whereas IL-4, IFN-gamma, and TNF down regulate TLR2 expression in monocytes (4).

Long Name

Toll-like Receptor 2

Alternate Names

CD282

Gene Symbol

TLR2

Additional TLR2 Products

Product Documents for TLR2 Antibody (11G5) - Azide Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for TLR2 Antibody (11G5) - Azide Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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