TLR2 Antibody (TL2.1) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB100-56722
Clone TL2.1 was used by HLDA to establish CD designation.
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse, Rat, Canine, Rabbit
Cited:
Human, Mouse, Rat, Canine, Rabbit
Applications
Validated:
Block/Neutralize, Dot Blot, ELISA, Flow (Cell Surface), Flow Cytometry, Functional, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, In vitro assay, Western Blot
Cited:
Block/Neutralize, Cytometric Bead Assay Standard, ELISA, Flow (Cell Surface), Flow Cytometry, Functional Assay, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, In vitro assay, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # TL2.1
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
This antibody was raised by immunizing mice with CHO cells transfected with human TLR2 cDNA (PMID: 10657659). The hybridoma supernatants were selected by flow cytometry.
Reactivity Notes
Mouse reactivity reported in scientific literature (PMID: 28219705, 29501923). Rat reactivity reported in scientific literature (PMID: 29501923).
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for TLR2 Antibody (TL2.1) - BSA Free
Flow Cytometry: TLR2 Antibody (TL2.1) - BSA Free [NB100-56722]
Flow Cytometry: TLR2 Antibody (TL2.1) [NB100-56722] - Analysis using the FITC conjugate of NB100-56726. Surface staining of stable HEK293/hTLR2 cells (IML-202, red) and vector control cells (IML-200, green) using TLR2 antibody at 1 ug/10^6 cells.Western Blot: TLR2 Antibody (TL2.1)BSA Free [NB100-56722]
Western Blot: TLR2 Antibody (TL2.1) [NB100-56722] - Expression of TLR2 was determined by western blotting. The immunoreactivity of the anti-TLR2 was confirmed with human intestinal lysate. Data are representative for analysis of >=2 independent experiments. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0060671) licensed under a CC-BY license.Western Blot: TLR2 Antibody (TL2.1)BSA Free [NB100-56722]
Western Blot: TLR2 Antibody (TL2.1) [NB100-56722] - Total protein from human THP-1 and U937 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-TLR2 (NB100-56722) in 5% BSA-TBST and detected with an anti-mouse HRP secondary antibody using NovaLume chemiluminescence detection reagent (NPB2-61915).Applications for TLR2 Antibody (TL2.1) - BSA Free
Application
Recommended Usage
Block/Neutralize
reported in scientific literature (PMID 25412776)
Dot Blot
reported in scientific literature (PMID 27248820)
ELISA
reported in multiple pieces of scientific literature
Flow (Cell Surface)
reported in scientific literature (PMID 24836676)
Flow Cytometry
1 ug/10^6 cells
Functional
reported in scientific literature (PMID 25412776)
Immunocytochemistry/ Immunofluorescence
1:10-1:500
Immunoprecipitation
2-5 ug
In vitro assay
reported in scientific literature (PMID 24512642)
Western Blot
1 - 2 ug/ml
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: TLR2
Long Name
Toll-like Receptor 2
Alternate Names
CD282
Gene Symbol
TLR2
Additional TLR2 Products
Product Documents for TLR2 Antibody (TL2.1) - BSA Free
Product Specific Notices for TLR2 Antibody (TL2.1) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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