TLR5 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-24827

Novus Biologicals, part of Bio-Techne
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NBP2-24827
NBP2-24827SS
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine

Cited:

Human, Mouse, Rat, Porcine

Applications

Validated:

Flow (Cell Surface), Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, In vitro assay, Western Blot

Cited:

Flow (Cell Surface), Flow Cytometry, IF/IHC, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all TLR5 Antibodies »

Product Specifications

Immunogen

This antibody was developed against KLH-conjugated synthetic peptide corresponding to a portion of human TLR5 found between amino acids 300-350.

Reactivity Notes

Porcine reactivity reported in scientific literature (PMID: 24097563).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TLR5 Antibody - BSA Free

Western Blot: TLR5 Antibody [NBP2-24827]

Western Blot: TLR5 Antibody [NBP2-24827]

Western Blot: TLR5 Antibody [NBP2-24827] - THP-1 cell lysate. Image from verified customer review.
Immunohistochemistry-Paraffin: TLR5 Antibody [NBP2-24827]

Immunohistochemistry-Paraffin: TLR5 Antibody [NBP2-24827]

Immunohistochemistry-Paraffin: TLR5 Antibody [NBP2-24827] - Analysis of TLR5 in FFPE human stomach tumor tissue using TLR5 antibody at 10 ug/mL.
Flow Cytometry: TLR5 Antibody [NBP2-24827]

Flow Cytometry: TLR5 Antibody [NBP2-24827]

Flow Cytometry: TLR5 Antibody [NBP2-24827] - Intracellular flow analysis of TLR5 in Balb/c mouse splenocytes using TLR5 antibody at 2 ug/10^6 cells. Shaded histogram: cells without antibody. Green: rabbit IgG isotype control. Red: TLR5 antibody. Goat anti-rabbit PE was used as secondary antibody.

Applications for TLR5 Antibody - BSA Free

Application
Recommended Usage

Flow (Cell Surface)

reported in scientific literature (PMID 24532644)

Flow Cytometry

1 - 2 ug/10^6 cells

Immunohistochemistry

reported in scientific literature (PMID 30716659)

In vitro assay

reported in scientific literature (PMID 26084721)

Western Blot

1 - 3 ug/mL

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: TLR5

The Toll like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules and could constitute an important and unrecognized component of innate immunity in humans. The TRL family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition. TLRs characterized so far activate the MyD88/interleukin 1 receptor-associated kinase (IRAK) signaling pathway. Toll-like receptor 5 (TLR5) expression is upregulated following exposure to bacteria or to the TLR5 agonist, flagellin. Gram-negative bacteria, stimulate monocyte/macrophage cells in a TLR5-specific, CD14-independent manner. The TLR5 receptor thus appears to be the principal means by which the innate immune system recognizes flagellated bacterial pathogens.

Long Name

Toll-like Receptor 5

Alternate Names

TIL-3

Gene Symbol

TLR5

UniProt

O60602

Additional TLR5 Products

Product Documents for TLR5 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for TLR5 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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