Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Flow (Cell Surface), Flow (Intracellular), Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Block/Neutralize, Flow (Cell Surface), Flow Cytometry, Flow Cytometry Control, IF/IHC, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 86B1153.2

Format

BSA Free

Concentration

1.0 mg/ml

View all TLR6 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a synthetic peptide corresponding to amino acids 408-424 of human TRL6.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for TLR6 Antibody (86B1153.2) - BSA Free

Immunohistochemistry-Paraffin: TLR6 Antibody (86B1153.2) - BSA Free [NB100-56536]

Immunohistochemistry-Paraffin: TLR6 Antibody (86B1153.2) - BSA Free [NB100-56536]

Immunohistochemistry-Paraffin: TLR6 Antibody (86B1153.2) [NB100-56536] - Tonsil probed with TLR6 antibody at 5 ug/ml. Novus's human tissue TMA was used for this test. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
Flow (Intracellular): TLR6 Antibody (86B1153.2) - BSA Free [NB100-56536]

Flow (Intracellular): TLR6 Antibody (86B1153.2) - BSA Free [NB100-56536]

Flow (Intracellular): TLR6 Antibody (86B1153.2) [NB100-56536] - Analysis using the PE conjugate of NB100-56536. Staining of TLR6 in Ramos cells using this antibody. 5 ug/10^6 cells. Shaded histogram represents Ramos cells without antibody; green represents isotype control (BD Pharmingen); red represents this antibody.
Flow Cytometry: TLR6 Antibody (86B1153.2) - BSA Free [NB100-56536]

Flow Cytometry: TLR6 Antibody (86B1153.2) - BSA Free [NB100-56536]

Flow Cytometry: TLR6 Antibody (86B1153.2) [NB100-56536] - Cell surface analysis of TLR6 antibody in 10^6 human monocytes using 0.5 ug of this antibody. Shaded histogram represents cells without antibody; green represents isotype control antibody ; red represents anti-TLR6 antibody. goat anti-mouse IgG PE conjugated secondary antibody was used.

Applications for TLR6 Antibody (86B1153.2) - BSA Free

Application
Recommended Usage

Flow (Cell Surface)

reported in scientific literature (PMID 24986635)

Flow Cytometry

3 ug/10-6 cells

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

5 ug/ml

Western Blot

reported in scientific literature (PMID 17332440)

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TLR6

The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules. The TRL family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition. TLRs characterized so far activate the MyD88/interleukin-1 receptor-associated kinase (IRAK) signaling pathway. Ten human homologs of TLRs (TLR1-10) have been described. The amino acid sequence of human TLR6 is most similar to hTLR1 with 69% identity at the amino acid level. Human TLR6 consists of a predicted 807 amino acids with a molecular weight of approximately 92 kDa. Human and mouse TLR6 share an amino acid identity of 73%. TLR6 activates both NF-kB and c-Jun N-terminal Kinase (JNK). The amino acids in the cytoplasmic domain of IL-1 receptor, which are critical for NF-kB activation, are conserved in TLR6. It has also been shown that TLR2-mediated response to a phenol-soluble factor from staphylococcus epidermidis is enhanced by TLR6. TLR6 is predominantly expressed in spleen, thymus, ovary, and lung.

Long Name

Toll-like Receptor 6

Alternate Names

CD286

Entrez Gene IDs

10333 (Human)

Gene Symbol

TLR6

UniProt

Q9Y2C9 (Human)

Additional TLR6 Products

Product Documents for TLR6 Antibody (86B1153.2) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for TLR6 Antibody (86B1153.2) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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