Key Product Details

Species Reactivity

Human

Applications

Flow (Cell Surface), Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 86B1153.2

Format

Azide Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all TLR6 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a synthetic peptide corresponding to amino acids 408-424 of human TRL6.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for TLR6 Antibody (86B1153.2) - Azide Free

Immunohistochemistry: TLR6 Antibody (86B1153.2) - Azide Free [NBP2-37704]

Immunohistochemistry: TLR6 Antibody (86B1153.2) - Azide Free [NBP2-37704]

Immunohistochemistry: TLR6 Antibody (86B1153.2) - Azide Free [NBP2-37704] - Tonsil probed with TLR6 antibody at 5 ug/ml. Novus's human tissue TMA was used for this test. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 m
Flow Cytometry: TLR6 Antibody (86B1153.2) - Azide Free [NBP2-37704]

Flow Cytometry: TLR6 Antibody (86B1153.2) - Azide Free [NBP2-37704]

Flow Cytometry: TLR6 Antibody (86B1153.2) - Azide Free [NBP2-37704] - Cell surface analysis of TLR6 antibody in 10^6 human monocytes using 0.5 ug of this antibody. Shaded histogram represents cells without antibody; green represents isotype control antibody ; red represents anti-TLR6 antibody. goat anti-mouse IgG PE conjugated secondary antibody was used. Image from the standard format of this antibody.

Applications for TLR6 Antibody (86B1153.2) - Azide Free

Application
Recommended Usage

Flow (Cell Surface)

0.5-1.0 ug/million cells

Immunohistochemistry

5 ug/ml

Immunohistochemistry-Paraffin

5 ug/ml

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TLR6

The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules. The TRL family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition. TLRs characterized so far activate the MyD88/interleukin-1 receptor-associated kinase (IRAK) signaling pathway. Ten human homologs of TLRs (TLR1-10) have been described. The amino acid sequence of human TLR6 is most similar to hTLR1 with 69% identity at the amino acid level. Human TLR6 consists of a predicted 807 amino acids with a molecular weight of approximately 92 kDa. Human and mouse TLR6 share an amino acid identity of 73%. TLR6 activates both NF-kB and c-Jun N-terminal Kinase (JNK). The amino acids in the cytoplasmic domain of IL-1 receptor, which are critical for NF-kB activation, are conserved in TLR6. It has also been shown that TLR2-mediated response to a phenol-soluble factor from staphylococcus epidermidis is enhanced by TLR6. TLR6 is predominantly expressed in spleen, thymus, ovary, and lung.

Long Name

Toll-like Receptor 6

Alternate Names

CD286

Gene Symbol

TLR6

Additional TLR6 Products

Product Documents for TLR6 Antibody (86B1153.2) - Azide Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for TLR6 Antibody (86B1153.2) - Azide Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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