TLR8 Antibody (44C143) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-24917
Clone 44C143 was used by HLDA to establish CD designation.
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Validated by
Orthogonal Validation, Biological Validation
Species Reactivity
Validated:
Human, Mouse, Rabbit
Cited:
Human, Mouse
Applications
Validated:
CyTOF-ready, Dot Blot, Flow (Cell Surface), Flow (Intracellular), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western, Western Blot
Cited:
Cytometric Bead Assay Standard, Flow - IC, Flow (Cell Surface), Flow Cytometry, Flow Cytometry Control, IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 44C143
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
This antibody was developed against a KLH-conjugated synthetic peptide of human TLR8, within amino acids 750-850.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Scientific Data Images for TLR8 Antibody (44C143) - BSA Free
Flow Cytometry: TLR8 Antibody (44C143) - BSA Free [NBP2-24917]
Flow Cytometry: TLR8 Antibody (44C143) [NBP2-24917] - Intracellular expression of TLR8 in monocyte-derived macrophages (MDMs). MDMs which were incubated with PE-conjugated anti-TLR8 or isotype antibody control. Intracellular expression was determined by flow cytometry. Representative profiles were similar in four independent experiments (n=4 subjects). Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0104039) licensed under a CC-BY license.Flow Cytometry: TLR8 Antibody (44C143) - BSA Free [NBP2-24917]
Flow Cytometry: TLR8 Antibody (44C143) [NBP2-24917] - Expression of TLR8 in Fravel, L363, OPM1, RPMI8226, XG1, and NCI-H929 as determined by flow cytometry. HCMLs were stained using an intracellular staining protocol with TLR8 antibodies followed by relevant secondary fluorescent-conjugated antibodies. Filled histograms (purple) represent the isotype controls and the open histograms (red) indicate TLR8. Data are representative for analysis of >=2 independent experiments. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0060671) licensed under a CC-BY license.Western Blot: TLR8 Antibody (44C143)BSA Free [NBP2-24917]
Western Blot: TLR8 Antibody (44C143) [NBP2-24917] - All the cell lines expressed mRNA for TLR8 (Figure 1) and displayed a strong expression of TLR8 protein (Figure 2). Lysates of human intestinal tissue and mouse splenocytes were used as positive controls. Beta-actin was served as loading control and was used to normalize expression levels between cells. Data are representative for analysis of >=2 independent experiments. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0060671) licensed under a CC-BY license.Applications for TLR8 Antibody (44C143) - BSA Free
Application
Recommended Usage
Dot Blot
reported in scientific literature (PMID 27248820)
Flow (Cell Surface)
reported in multiple pieces of scientific literature
Flow (Intracellular)
reported in reported in multiple pieces of scientific literature (PMID 24836676)
Flow Cytometry
0.5-1 ug/10^6 cells
Immunohistochemistry-Paraffin
5 ug/ml
Simple Western
20 ug/ml
Western Blot
1-3 ug/ml
Application Notes
Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min. This antibody is CyTOF ready.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS pH 7.4
Format
BSA Free
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: TLR8
TLR8 is highly similar to TLR7 and both pathways are mediated by the adapter protein MyD88 to signal through IFN regulatory factor 7 (IRF7) and nuclear factor (NF)-kappaB (1-3,5). However, TLR7 recognizes guanosine and GU-rich ssRNA, while TLR8 recognizes uridine and AU-rich sequences (2,5). TLR7/TLR8 agonists, including derivatives of the immunostimulatory imiquimod, have been shown to be a promising cancer therapy capable of providing anticancer signals to antigen presenting cells (APCs), with many agonists being tested in both pre-clinical and clinical trials (6). Similarly, studies suggest that agonists for TLR8, in combination with other individual TLR agonists and antagonists, may also be useful for treating inflammatory allergic diseases, such as allergic rhinitis (7).
References
1. Sakaniwa, K., & Shimizu, T. (2020). Targeting the innate immune receptor TLR8 using small-molecule agents. Acta crystallographica. Section D, Structural biology, 76(Pt 7). https://doi.org/10.1107/S2059798320006518
2. Cervantes, J. L., Weinerman, B., Basole, C., & Salazar, J. C. (2012). TLR8: the forgotten relative revindicated. Cellular & molecular immunology. https://doi.org/10.1038/cmi.2012.38
3. Ohto, U., Tanji, H., & Shimizu, T. (2014). Structure and function of toll-like receptor 8. Microbes and infection. https://doi.org/10.1016/j.micinf.2014.01.007
4. Uniprot (Q9NR97)
5. Jannuzzi, G. P., de Almeida, J., Paulo, L., de Almeida, S. R., & Ferreira, K. S. (2020). Intracellular PRRs Activation in Targeting the Immune Response Against Fungal Infections. Frontiers in cellular and infection microbiology. https://doi.org/10.3389/fcimb.2020.591970
6. Frega, G., Wu, Q., Le Naour, J., Vacchelli, E., Galluzzi, L., Kroemer, G., & Kepp, O. (2020). Trial Watch: experimental TLR7/TLR8 agonists for oncological indications. Oncoimmunology. https://doi.org/10.1080/2162402X.2020.1796002
7. Golshiri-Isfahani, A., Amizadeh, M., & Arababadi, M. K. (2018). The roles of toll like receptor 3, 7 and 8 in allergic rhinitis pathogenesis. Allergologia et immunopathologia. https://doi.org/10.1016/j.aller.2017.09.026
Long Name
Toll-like Receptor 8
Alternate Names
CD288
Gene Symbol
TLR8
Additional TLR8 Products
Product Documents for TLR8 Antibody (44C143) - BSA Free
Product Specific Notices for TLR8 Antibody (44C143) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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