TLR9 Antibody (5G5)
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-51156
Key Product Details
Species Reactivity
Human, Mouse, Canine
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 5G5
Concentration
0.1 mg/ml
Product Specifications
Immunogen
Purified fusion protein of extracellular domain of human TLR9 (AA 1-815) and human IgGFc
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Description
Positive Control: RAW264.7 macrophages stimulated with IFNg
Scientific Data Images for TLR9 Antibody (5G5)
Flow Cytometry: TLR9 Antibody (5G5) [NBP1-51156]
Flow Cytometry: TLR9 Antibody (5G5) [NBP1-51156] - THP1 cells were incubated with IgG2a isptype controle Cells (140000) were permeabilized with saponin and stained with 0.4 ug 5G5Flow Cytometry: TLR9 Antibody (5G5) [NBP1-51156]
Flow Cytometry: TLR9 Antibody (5G5) [NBP1-51156] - THP1 cells were incubated with IgG2a isptype controle (A) or a-TLR9 5G5 mAbApplications for TLR9 Antibody (5G5)
Application
Recommended Usage
Flow Cytometry
1:50
Immunocytochemistry/ Immunofluorescence
4ug/400ul
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Frozen
1:100
Immunohistochemistry-Paraffin
1:50
Western Blot
2 ug/ml
Application Notes
IHC-Frozen: 10um sections were fixed with acetone for 10 minutes. PBS washed sections were incubated with 5G5 1:100 in 1% BSA for 30 minutes at RT. IF: cells were fixed with 2% formalin for 15 minutes at RT and permeabilized with a mAb (4ug/400ul) containing buffer (PBS, 0.2% BSA, 0.2% saponin) for 1 hour. FC: RAW264.7 cells were fixed for 15 minutes with 4% formalin and permeabilized (PBS, 0.5%BSA, 0,5% saponin) at RT. IHC-Paraffin: paraffin embedded tissues 5um sections were made. After antigen retrieval (0.01mol/l, pH6 sodium citrate) and quenching of endogenous peroxidase, sections were blocked with 0.5% ovalbumin and 0.1% gelatin for 20 minutes at RT. Sections were incubated with 5G5 for 1 hour at 37C. WB: reduced lysates were resolved by 10% SDS-PAGE and blotted on nitrocellulose. After blocking with 5% skimmed milk TLR9 was detected with 2 ug/ml 5G5
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
PBS, containing 0.1% BSA
Preservative
0.02% Sodium Azide
Concentration
0.1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: TLR9
Long Name
Toll-like Receptor 9
Alternate Names
CD289
Gene Symbol
TLR9
Additional TLR9 Products
Product Documents for TLR9 Antibody (5G5)
Product Specific Notices for TLR9 Antibody (5G5)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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