Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI10G3

Concentration

0.4 mg/ml

View all TMEM100 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human TMEM100 (NP_060756) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Specificity

This antibody is specific for Homo sapiens transmembrane protein 100 (TMEM100), transcript variant 2.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

14.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for TMEM100 Antibody (OTI10G3)

Western Blot: TMEM100 Antibody (OTI10G3) [NBP1-48024]

Western Blot: TMEM100 Antibody (OTI10G3) [NBP1-48024]

Western Blot: TMEM100 Antibody (10G3) [NBP1-48024] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TMEM100 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TMEM100.
Immunocytochemistry/Immunofluorescence: TMEM100 Antibody (10G3) [NBP1-48024] Staining of COS7 cells transiently transfected by pCMV6-ENTRY TMEM100.
Immunohistochemistry-Paraffin: TMEM100 Antibody (OTI10G3) [NBP1-48024]

Immunohistochemistry-Paraffin: TMEM100 Antibody (OTI10G3) [NBP1-48024]

Immunohistochemistry-Paraffin: TMEM100 Antibody (10G3) [NBP1-48024] - Staining of paraffin-embedded Human pancreas tissue using anti-TMEM100 mouse monoclonal antibody.
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Applications for TMEM100 Antibody (OTI10G3)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50

Immunohistochemistry-Paraffin

1:50

Western Blot

1:500

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.4 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: TMEM100

Alternate Names

FLJ10970, FLJ37856, transmembrane protein 100

Entrez Gene IDs

55273 (Human)

Gene Symbol

TMEM100

Additional TMEM100 Products

Product Documents for TMEM100 Antibody (OTI10G3)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for TMEM100 Antibody (OTI10G3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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