Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI10G3

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all TMEM100 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human TMEM100 (NP_060756) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

14.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for TMEM100 Antibody (OTI10G3) - Azide and BSA Free

Western Blot: TMEM100 Antibody (OTI10G3)Azide and BSA Free [NBP2-74545]

Western Blot: TMEM100 Antibody (OTI10G3)Azide and BSA Free [NBP2-74545]

Western Blot: TMEM100 Antibody (OTI10G3) - Azide and BSA Free [NBP2-74545] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TMEM100 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TMEM100.
Immunocytochemistry/ Immunofluorescence: TMEM100 Antibody (OTI10G3) - Azide and BSA Free [NBP2-74545]

Immunocytochemistry/ Immunofluorescence: TMEM100 Antibody (OTI10G3) - Azide and BSA Free [NBP2-74545]

Immunocytochemistry/Immunofluorescence: TMEM100 Antibody (OTI10G3) - Azide and BSA Free [NBP2-74545] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY TMEM100.
Immunohistochemistry: TMEM100 Antibody (OTI10G3) - Azide and BSA Free [NBP2-74545]

Immunohistochemistry: TMEM100 Antibody (OTI10G3) - Azide and BSA Free [NBP2-74545]

Immunohistochemistry: TMEM100 Antibody (OTI10G3) - Azide and BSA Free [NBP2-74545] - Staining of paraffin-embedded Human Ovary tissue using anti-TMEM100 mouse monoclonal antibody.
View 4 more images

Applications for TMEM100 Antibody (OTI10G3) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50

Western Blot

1:500

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: TMEM100

Alternate Names

FLJ10970, FLJ37856, transmembrane protein 100

Gene Symbol

TMEM100

Additional TMEM100 Products

Product Documents for TMEM100 Antibody (OTI10G3) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for TMEM100 Antibody (OTI10G3) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.