TMEM119 Antibody (CL8714) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-43823
![TMEM119 Antibody (CL8714) - Azide and BSA Free Immunohistochemistry-Paraffin: TMEM119 Antibody [NBP3-43823]](https://resources.bio-techne.com/images/products/nbp3-43823_mouse-tmem119-mab-cl8714-azide-and-bsa-free-28220251391955.jpg "Immunohistochemistry-Paraffin: TMEM119 Antibody [NBP3-43823]")
![TMEM119 Antibody (CL8714) - Azide and BSA Free Immunohistochemistry-Paraffin: TMEM119 Antibody [NBP3-43823]](https://resources.bio-techne.com/images/products/nbp3-43823_mouse-tmem119-mab-cl8714-azide-and-bsa-free-28220251211186.jpg "Immunohistochemistry-Paraffin: TMEM119 Antibody [NBP3-43823]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # CL8714
Format
Azide and BSA Free
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
This antibody was generated using a recombinant protein sequence of Q4V9L6, with the exact immunogen sequence remaining proprietary.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for TMEM119 Antibody (CL8714) - Azide and BSA Free
![TMEM119 Antibody (CL8714) - Azide and BSA Free Immunohistochemistry-Paraffin: TMEM119 Antibody [NBP3-43823]](https://resources.bio-techne.com/images/products/nbp3-43823_mouse-tmem119-mab-cl8714-azide-and-bsa-free-28220251273753.jpg "Immunohistochemistry-Paraffin: TMEM119 Antibody [NBP3-43823]")
Immunohistochemistry-Paraffin: TMEM119 Antibody [NBP3-43823]
Staining of human cerebellum shows strong membranous positivity in microglia.![TMEM119 Antibody (CL8714) - Azide and BSA Free Immunohistochemistry-Paraffin: TMEM119 Antibody [NBP3-43823]](https://resources.bio-techne.com/images/products/nbp3-43823_mouse-tmem119-mab-cl8714-azide-and-bsa-free-282202512405521.jpg "Immunohistochemistry-Paraffin: TMEM119 Antibody [NBP3-43823]")
Immunohistochemistry-Paraffin: TMEM119 Antibody [NBP3-43823]
Staining of human cerebral cortex shows strong membranous positivity in microglia.![TMEM119 Antibody (CL8714) - Azide and BSA Free Immunohistochemistry-Paraffin: TMEM119 Antibody [NBP3-43823]](https://resources.bio-techne.com/images/products/nbp3-43823_mouse-tmem119-mab-cl8714-azide-and-bsa-free-28220251353248.jpg "Immunohistochemistry-Paraffin: TMEM119 Antibody [NBP3-43823]")
![TMEM119 Antibody (CL8714) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: TMEM119 Antibody [NBP3-43823]](https://resources.bio-techne.com/images/products/nbp3-43823_mouse-tmem119-mab-cl8714-azide-and-bsa-free-28220251339153.jpg "Immunocytochemistry/ Immunofluorescence: TMEM119 Antibody [NBP3-43823]")
![TMEM119 Antibody (CL8714) - Azide and BSA Free Western Blot: TMEM119 Antibody [NBP3-43823]](https://resources.bio-techne.com/images/products/nbp3-43823_mouse-tmem119-mab-cl8714-azide-and-bsa-free-28220251339127.jpg "Western Blot: TMEM119 Antibody [NBP3-43823]")
Applications for TMEM119 Antibody (CL8714) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
2-10 ug/ml
Immunohistochemistry-Paraffin
1:5000 - 1:10000
Western Blot
1 ug/ml
Application Notes
For IHC, HIER pH 6 retrieval is recommended. Immunocytochemistry/ Immunofluorescence/IF Fixation/Permeabilization: PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Protein A purified
Reconstitution
Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.
Formulation
Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: TMEM119
ATF4 is an approximately 38 kDa member of the bzip family of transcription factors. It plays an important role autophagy, the unfolded protein response, and the response to oxygen and nutrient deprivation. These functions are regulated by the dimerization of ATF4 with the leucine zipper protein FIAT. ATF4 contains a basic DNA-binding domain (aa 280-300) and a leucine zipper domain (aa 306-334).
Long Name
Transmembrane Protein 119
Alternate Names
OBIF
Gene Symbol
TMEM119
Additional TMEM119 Products
Product Documents for TMEM119 Antibody (CL8714) - Azide and BSA Free
Product Specific Notices for TMEM119 Antibody (CL8714) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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