Tmp21/p23 Antibody (PSH01-40)

Western Blot: Tmp21/p23 Antibody (PSH01-40) [NBP3-33030]

Western Blot: Tmp21/p23 Antibody (PSH01-40) [NBP3-33030] - Western blot analysis of Tmp21/p23 on different lysates with Rabbit anti-Tmp21/p23 antibody (NBP3-33030) at 1/5,000 dilution.

Lane 1: HeLa cell lysate (20 ug/Lane)
Lane 2: 293T cell lysate (20 ug/Lane)
Lane 3: Raji cell lysate (20 ug/Lane)
Lane 4: Jurkat cell lysate (20 ug/Lane)
Lane 5: HepG2 cell lysate (20 ug/Lane)
Lane 6: Ramos cell lysate (20 ug/Lane)
Lane 7: U-87 MG cell lysate (20 ug/Lane)
Lane 8: RAW264.7 cell lysate (20 ug/Lane)
Lane 9: Neuro-2a cell lysate (20 ug/Lane)
Lane 10: PC-12 cell lysate (20 ug/Lane)
Lane 11: Mouse pancreas tissue lysate (40 ug/Lane)
Lane 12: Mouse liver tissue lysate (40 ug/Lane)
Lane 13: Rat pancreas tissue lysate (40 ug/Lane)
Lane 14: Rat liver tissue lysate (40 ug/Lane)

Predicted band size: 25 kDa
Observed band size: 19 kDa

Exposure time: 2 minutes 13 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33030) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: Tmp21/p23 Antibody (PSH01-40) [NBP3-33030]

Immunohistochemistry: Tmp21/p23 Antibody (PSH01-40) [NBP3-33030] - Immunofluorescence analysis of paraffin-embedded human breast tissue labeling Tmp21/p23 with Rabbit anti-Tmp21/p23 antibody (NBP3-33030) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-33030, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

Immunohistochemistry: Tmp21/p23 Antibody (PSH01-40) [NBP3-33030]

Immunohistochemistry: Tmp21/p23 Antibody (PSH01-40) [NBP3-33030] - Immunohistochemical analysis of paraffin-embedded human breast tissue with Rabbit anti-Tmp21/p23 antibody (NBP3-33030) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33030) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.