Tmp21/p23 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB110-57587

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse, Rat, Bovine, Primate, Rabbit, Xenopus

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

0.95 mg/ml

View all Tmp21/p23 Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to an internal sequence of the human TMP21 protein (within residues 50-150). [Swiss-Prot# P49755]

Localization

Type I membrane. Golgi cisternae.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Tmp21/p23 Antibody - BSA Free

Knockdown Validated: Tmp21/p23 Antibody - BSA Free [NB110-57587]

Knockdown Validated: Tmp21/p23 Antibody - BSA Free [NB110-57587]

Knockdown Validated: Tmp21/p23 Antibody [NB110-57587] - Western blot shows lysates of HEK293T human embryonic kidney parental cell line and TMP21/TMED10 knockout (KO) HEK293T cell line. PVDF membrane was probed with 2.0 ug/ml of Rabbit Anti-Human TMP21/TMED10 Polyclonal Antibody (Catalog # NB110-57587) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for TMP21/TMED10 at approximately 25 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in the knockout HEK293T cell line. This experiment was conducted under reduc
Western Blot: Tmp21/p23 AntibodyBSA Free [NB110-57587]

Western Blot: Tmp21/p23 AntibodyBSA Free [NB110-57587]

Western Blot: TMP21 Antibody [NB110-57587] - Detection of TMP21 in human liver lysate using NB110-57587.
Immunohistochemistry-Paraffin: Tmp21/p23 Antibody - BSA Free [NB110-57587]

Immunohistochemistry-Paraffin: Tmp21/p23 Antibody - BSA Free [NB110-57587]

Immunohistochemistry-Paraffin: Tmp21/p23 Antibody [NB110-57587] - Tmp21/p23 was detected in immersion fixed paraffin-embedded sections of human liver using Rabbit Anti-Human TMP21/p23 polyclonal Antibody (Catalog # NB110-57587) at 1 ug/ml for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm in hepatocytes.

Applications for Tmp21/p23 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1 ug/ml

Western Blot

2 ug/ml
Application Notes
This TMP21 antibody is useful for Immunohistochemistry and Western blot analysis, where a band is seen at ~21 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS and 30% Glycerol

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

0.95 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Tmp21/p23

TMP21 (transmembrane protein Tmp21) is a type I transmembrane protein with large luminal domain belonging to p24 endoplasmic reticulum/Golgi cargo family and play key roles in protein transport, membrane structure organization and secretory pathway. TMP21 is ubiquitously expressed with highest protein levels observed in pancreas and intestines. In addition to its well established role in vesicle formation and cargo transport in ER/Golgi, TMP21 has been implicated in recruitment of small GTPase ADP-ribosylation factor 1 to Golgi apparatus and ADP-ribosylation factor 1-dependent assembly of actin in the Golgi. TMP21 forms a component of presenilin complex at the plasma membranes and modulates gamma -secretase cleavage. The cytoplasmic tails of p24 family interacts with coatomer (major component of coat protein complex I or COP-I), to facilitate transport between ER and Golgi. TMP21 cycles through the early secretory pathway between intermediate and cis-Golgi compartments, and is essential for proper organization of the Golgi apparatus. TMP21 also plays an essential and non-redundant role in earliest stages of mammalian development.

Long Name

21 kDa Transmembrane Trafficking Protein

Alternate Names

p24delta, S31I125, S31III125, TMED10

Gene Symbol

TMED10

Additional Tmp21/p23 Products

Product Documents for Tmp21/p23 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Tmp21/p23 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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