TNF-alpha Antibody (R023) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-90547
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Mouse
Applications
Neutralization
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # R023
Format
Azide and BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was obtained from a rabbit immunized with purified, recombinant Mouse TNF-alpha(Leu80-Leu235; Accession#: NP_038721.1).
Specificity
Has cross-reactivity in ELISA with: Human TNF-alpha/TNFA
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for TNF-alpha Antibody (R023) - Azide and BSA Free
![Neutralization: TNF-alpha Antibody (R023) [NBP2-90547]](https://resources.bio-techne.com/images/products/TNF-alpha-Antibody-R023-Neutralization-NBP2-90547-img0001.jpg "Neutralization: TNF-alpha Antibody (R023) [NBP2-90547]")
Neutralization: TNF-alpha Antibody (R023) [NBP2-90547]
Neutralization: TNF-alpha Antibody (R023) [NBP2-90547] - Recombinant Mouse TNF-alpha induces cytotoxicity in the L929 mouse fibroblast cell line in a dose dependent manner. Cytotoxicity elicited by Recombinant Mouse TNF-alpha (0.1 ng/mL) is neutralized by increasing concentrations of Mouse TNF-alpha Monoclonal Antibody. The ND50 is typically 75-125 ng/mL in the presence of the metabolic inhibit or actinomycin D (1 ug/mL).Applications for TNF-alpha Antibody (R023) - Azide and BSA Free
Application
Recommended Usage
Neutralization
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
0.2 um filtered solution in Histidine and Arginine buffer, 120mM NaCl, 0.02% Tween 80, pH6.0
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: TNF-alpha
TNF-alpha is critical for normal immune response; however, dysregulation of TNF-alpha production can result in various pathologies (2,4,5). Excessive production of pro-inflammatory cytokines including interleukin 1 (IL-1), IL-6, and TNF-alpha has been implicated in an array of autoimmune diseases like rheumatoid arthritis (RA), inflammatory bowel disease (IBD), and psoriasis (2,4,5). Anti-TNF monoclonal antibodies, including Infliximab, and soluble TNFR have been approved for the treatment of autoimmune and TNF-mediated diseases (5). Additionally, data suggests that TNF inhibitors can be beneficial for treating patients experiencing immune-related adverse events associated with immune checkpoint inhibitor cancer treatment (6).
References
1. Holbrook J, Lara-Reyna S, Jarosz-Griffiths H, McDermott M. Tumour necrosis factor signalling in health and disease. F1000Res. 2019;8:F1000 Faculty Rev-111. https://doi.org/10.12688/f1000research.17023.1
2. Jang DI, Lee AH, Shin HY, et al. The Role of Tumor Necrosis Factor Alpha (TNF-alpha) in Autoimmune Disease and Current TNF-alpha Inhibitors in Therapeutics. Int J Mol Sci. 2021;22(5):2719. https://doi.org/10.3390/ijms22052719
3. Horiuchi T, Mitoma H, Harashima S, Tsukamoto H, Shimoda T. Transmembrane TNF-alpha: structure, function and interaction with anti-TNF agents. Rheumatology (Oxford). 2010;49(7):1215-1228. https://doi.org/10.1093/rheumatology/keq031
4. Webster JD, Vucic D. The Balance of TNF Mediated Pathways Regulates Inflammatory Cell Death Signaling in Healthy and Diseased Tissues. Front Cell Dev Biol. 2020;8:365. https://doi.org/10.3389/fcell.2020.00365
5. Kalliolias GD, Ivashkiv LB. TNF biology, pathogenic mechanisms and emerging therapeutic strategies. Nat Rev Rheumatol. 2016; 12(1):49-62. https://doi.org/10.1038/nrrheum.2015.169
6. Chen AY, Wolchok JD, Bass AR. TNF in the era of immune checkpoint inhibitors: friend or foe?. Nat Rev Rheumatol. 2021;17(4):213-223. doi:10.1038/s41584-021-00584-4
Long Name
Tumor Necrosis Factor alpha
Alternate Names
Cachetin, DIF, TNF, TNF-A, TNFA, TNFalpha, TNFG1F, TNFSF1A, TNFSF2
Gene Symbol
TNF
Additional TNF-alpha Products
Product Documents for TNF-alpha Antibody (R023) - Azide and BSA Free
Product Specific Notices for TNF-alpha Antibody (R023) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...