TNF-alpha Antibody (Z74P2C5*A12) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-61611

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Fish

Applications

ELISA, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG Clone # Z74P2C5*A12

Format

BSA Free

Concentration

1 mg/ml

View all TNF-alpha Antibodies »

Product Specifications

Immunogen

Ovalbumin-conjugated synthetic peptide

Reactivity Notes

Salmon and Rainbow Trout

Clonality

Monoclonal

Host

Mouse

Isotype

IgG

Description

The clone number is listed as Z74P2C5*A12 in the scientific literature.

Scientific Data Images for TNF-alpha Antibody (Z74P2C5*A12) - BSA Free

Immunohistochemistry: TNF-alpha Antibody (Z74P2C5*A12) - BSA Free [NBP2-61611]

Immunohistochemistry: TNF-alpha Antibody (Z74P2C5*A12) - BSA Free [NBP2-61611]

Immunohistochemistry: TNF alpha Variant Antibody (Z74P2C5*A12) [NBP2-61611] - Immunohistochemistry image showing positive staining of TNF alpha Variant Antibody (Z74P2C5*A12) in adipose tissue vaccinated rainbow trout. IHC was performed on formalin-fixed, paraffin-embedded tissue sections. Antigen retrieval step is required, which involves microwaving tissue for 20 minutes @ 800W in 10 mM citrate buffer (pH 6.0).
TNF-alpha Antibody (Z74P2C5*A12) - BSA Free

Immunohistochemistry: TNF-alpha Antibody (Z74P2C5*A12) - BSA Free [NBP2-61611] -

Immunohistochemistry: TNF-alpha Antibody (Z74P2C5*A12) - BSA Free [NBP2-61611] - Stained sections of fish intestine and liver of the control group showed negative staining reactions in all the examined parts of the intestinal and hepatic tissue (black and red arrows) against caspase-3 (NB600-1235) and TNF-alpha (NBP2-61611) antibodies. Nearly 10% of hepatic portal cells reacted positively to caspase-3. Scale bars = 10 um. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35624801/) licensed under a CC-BY license.

Applications for TNF-alpha Antibody (Z74P2C5*A12) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunohistochemistry

1:10-1:500

Western Blot

1:100-1:2000
Application Notes
Positive control(s): Western Blot: recombinant TNF-alpha 1 and TNF-alpha 1. IHC: formalin-fixed, paraffin-embedded adipose fish tissues.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TNF-alpha

Tumor necrosis factor (TNF)-alpha is a pro-inflammatory cytokine belonging to the TNF superfamily that is secreted by monocytes/macrophages, T cell, and natural killer (NK), among others (1). TNF-alpha is synthesized as a 233 amino acid (aa) transmembrane protein (mTNF-alpha) with a theoretical molecular weight (MW) of 26 kDa (1,2) that forms a homotrimer. mTNF-alpha is cleaved by TNF-alpha converting enzyme (TACE) and released in its 157 aa, 17 kDa soluble form (sTNF-alpha) (1-5). Both mTNF-alpha and sTNF-alpha are capable of binding type 1 TNF receptors (TNFR1), whereas mTNF-alpha predominately binds to TNFR2 (1,2). TNF-alpha binding to its receptors causes receptor recruitment of adaptor proteins, formation of signaling complexes, and downstream signaling cascades (e.g. MAPK, NF-kappaB, and Caspase-8), leading to distinct cellular responses such as survival, proliferation, inflammation, necroptosis, and apoptosis (1-5).

TNF-alpha is critical for normal immune response; however, dysregulation of TNF-alpha production can result in various pathologies (2,4,5). Excessive production of pro-inflammatory cytokines including interleukin 1 (IL-1), IL-6, and TNF-alpha has been implicated in an array of autoimmune diseases like rheumatoid arthritis (RA), inflammatory bowel disease (IBD), and psoriasis (2,4,5). Anti-TNF monoclonal antibodies, including Infliximab, and soluble TNFR have been approved for the treatment of autoimmune and TNF-mediated diseases (5). Additionally, data suggests that TNF inhibitors can be beneficial for treating patients experiencing immune-related adverse events associated with immune checkpoint inhibitor cancer treatment (6).

References

1. Holbrook J, Lara-Reyna S, Jarosz-Griffiths H, McDermott M. Tumour necrosis factor signalling in health and disease. F1000Res. 2019;8:F1000 Faculty Rev-111. https://doi.org/10.12688/f1000research.17023.1

2. Jang DI, Lee AH, Shin HY, et al. The Role of Tumor Necrosis Factor Alpha (TNF-alpha) in Autoimmune Disease and Current TNF-alpha Inhibitors in Therapeutics. Int J Mol Sci. 2021;22(5):2719. https://doi.org/10.3390/ijms22052719

3. Horiuchi T, Mitoma H, Harashima S, Tsukamoto H, Shimoda T. Transmembrane TNF-alpha: structure, function and interaction with anti-TNF agents. Rheumatology (Oxford). 2010;49(7):1215-1228. https://doi.org/10.1093/rheumatology/keq031

4. Webster JD, Vucic D. The Balance of TNF Mediated Pathways Regulates Inflammatory Cell Death Signaling in Healthy and Diseased Tissues. Front Cell Dev Biol. 2020;8:365. https://doi.org/10.3389/fcell.2020.00365

5. Kalliolias GD, Ivashkiv LB. TNF biology, pathogenic mechanisms and emerging therapeutic strategies. Nat Rev Rheumatol. 2016; 12(1):49-62. https://doi.org/10.1038/nrrheum.2015.169

6. Chen AY, Wolchok JD, Bass AR. TNF in the era of immune checkpoint inhibitors: friend or foe?. Nat Rev Rheumatol. 2021;17(4):213-223. doi:10.1038/s41584-021-00584-4

Long Name

Tumor Necrosis Factor alpha

Alternate Names

Cachetin, DIF, TNF, TNF-A, TNFA, TNFalpha, TNFG1F, TNFSF1A, TNFSF2

Gene Symbol

TNF

Additional TNF-alpha Products

Product Documents for TNF-alpha Antibody (Z74P2C5*A12) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for TNF-alpha Antibody (Z74P2C5*A12) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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