TOM70 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB110-58347

Novus Biologicals, part of Bio-Techne
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NB110-58347
NB110-58347SS
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Rabbit

Cited:

Human, Rabbit

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Simple Western, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all TOM70 Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to a region within residues 1-100 of the human TOM70 protein. [Swiss-Prot# O94826]

Reactivity Notes

Rabbit reactivity reported in scientific literature (PMID: 22292033).

Localization

Mitochondrial; multi-pass membrane.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

67 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for TOM70 Antibody

Western Blot: TOM70 Antibody [NB110-58347]

Western Blot: TOM70 Antibody [NB110-58347]

Western Blot: TOM70 Antibody [NB110-58347] - Detection of TOM70 in MCF7 mitochondrial lysate
Immunocytochemistry/ Immunofluorescence: TOM70 Antibody [NB110-58347]

Immunocytochemistry/ Immunofluorescence: TOM70 Antibody [NB110-58347]

Immunocytochemistry/Immunofluorescence: TOM70 Antibody [NB110-58347] - TOM70 antibody was tested in MCF7 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
Simple Western: TOM70 Antibody [NB110-58347]

Simple Western: TOM70 Antibody [NB110-58347]

Simple Western: TOM70 Antibody [NB110-58347] - Simple Western lane view shows a specific band for TOM70 in 0.5 mg/ml of MCF-7 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Applications for TOM70 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Simple Western

1:50

Western Blot

1:500
Application Notes
This TOM70 antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a band is seen at approx. 67 kDa. In ICC/IF mitochondrial staining was observed in MCF7 cells.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: TOM70

Most of mitochondrial proteins are synthesized by cytosolic ribosomes followed by import into mitochondria post-translationally with the help of cytosolic chaperones as well as mitochondrial translocators (import components). Translocase of the outer membrane (TOM) complex (TOMM5, TOMM6, TOMM7, TOMM20, TOMM22, TOMM40 and TOMM70) is the proteinaceous machinery that contains surface receptors for pre-protein recognition and serve as main entry gateway into mitochondria. Mitochondrial targeting requires different cytosolic factors, in particular the molecular chaperones (Hsc70/Hsp70 and Hsp90) whose activity occur in coordinated cycles of ATP hydrolysis/substrate binding and are regulated by a number of co-chaperone proteins including TOM70, a member of TPR (tetratricopeptide repeat) co-chaperone family. TOM70 contains a conserved TPR clamp domain for interaction with Hsc70 and Hsp90, and such interaction is essential for import process initiation. TOMM70 is a single-pass membrane protein localized in mitochondrion outer membrane and is ubiquitously expressed in various tissues where it acts as a receptor that accelerates the import of all mitochondrial precursor proteins. In addition to importing inner membrane carrier proteins, Tom70 is involved in targeting Mcl-1 to outer membrane via an internal EELD sequence.

Alternate Names

FLJ90470, KIAA0719, mitochondrial import receptor subunit TOM70, Mitochondrial precursor proteins import receptor, TOM70, Translocase of outer membrane 70 kDa subunit, translocase of outer mitochondrial membrane 70 (yeast) homolog A, translocase of outer mitochondrial membrane 70 homolog A (S. cerevisiae), translocase of outer mitochondrial membrane 70 homolog A (yeast)

Gene Symbol

TOMM70

Additional TOM70 Products

Product Documents for TOM70 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for TOM70 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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