TOMM20 Antibody (1B2) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-60248
![Western Blot: TOMM20 Antibody (1B2)BSA Free [NBP2-60248]](https://resources.bio-techne.com/images/products/TOMM20-Antibody-1B2-Western-Blot-NBP2-60248-img0001.jpg "Western Blot: TOMM20 Antibody (1B2)BSA Free [NBP2-60248]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Applications
ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 1B2
Format
BSA Free
Concentration
1 mg/ml
Product Summary for TOMM20 Antibody (1B2) - BSA Free
Immunogen
Recombinant human Tomm20 (25-145aa) purified from E. coli
Reactivity Notes
Mouse reactivity reported from a verified customer review.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Scientific Data Images for TOMM20 Antibody (1B2) - BSA Free
Western Blot: TOMM20 Antibody (1B2)BSA Free [NBP2-60248]
Western Blot: TOMM20 Antibody (1B2) [NBP2-60248] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Tomm20 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: HeLa cell lysateLane 2.: HepG2 cell lysate Lane 3.: A431 cell lysate Lane 4.: K562 cell lysate Lane 5.: A549 cell lysate Lane 6.: 293T cell lysate Lane 7.: MCF7 cell lysate Lane 8.: SK-OV-3 cell lysate Lane 9.: PC3 cell lysate![Immunocytochemistry/ Immunofluorescence: TOMM20 Antibody (1B2) - BSA Free [NBP2-60248]](https://resources.bio-techne.com/images/products/TOMM20-Antibody-1B2-Immunocytochemistry-Immunofluorescence-NBP2-60248-img0002.jpg "Immunocytochemistry/ Immunofluorescence: TOMM20 Antibody (1B2) - BSA Free [NBP2-60248]")
Immunocytochemistry/ Immunofluorescence: TOMM20 Antibody (1B2) - BSA Free [NBP2-60248]
Immunocytochemistry/Immunofluorescence: TOMM20 Antibody (1B2) [NBP2-60248] - Staining in HeLa cells. The secondary antibody (green) was Alexa Fluor 488. DAPI was stained the cell nucleus (blue).![Flow Cytometry: TOMM20 Antibody (1B2) - BSA Free [NBP2-60248]](https://resources.bio-techne.com/images/products/TOMM20-Antibody-1B2-Flow-Cytometry-NBP2-60248-img0005.jpg "Flow Cytometry: TOMM20 Antibody (1B2) - BSA Free [NBP2-60248]")
Flow Cytometry: TOMM20 Antibody (1B2) - BSA Free [NBP2-60248]
Flow Cytometry: TOMM20 Antibody (1B2) [NBP2-60248] - Analysis of Tomm20 in HeLa cells. The cell was stained, at 2-5ug for 1x106cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray)Applications for TOMM20 Antibody (1B2) - BSA Free
Application
Recommended Usage
ELISA
1:100 - 1:2000
Flow Cytometry
2-5 ug
Immunocytochemistry/ Immunofluorescence
1:100
Western Blot
1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using NBP2-60248 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.4), 10% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: TOMM20
Long Name
TOMM20
Alternate Names
MAS20, mitochondrial 20 kDa outer membrane protein, mitochondrial import receptor subunit TOM20 homolo, MOM19, outer mitochondrial membrane receptor Tom20, TOM20, translocase of outer mitochondrial membrane 20 hom
Gene Symbol
TOMM20
Additional TOMM20 Products
Product Documents for TOMM20 Antibody (1B2) - BSA Free
Product Specific Notices for TOMM20 Antibody (1B2) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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