TOP2A Antibody (1E2)

Novus Biologicals, part of Bio-Techne | Catalog # H00007153-M01

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Product Datasheet / COA / SDS

Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ kappa Clone # 1E2

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

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Product Specifications

Immunogen

TOP2A (NP_001058, 1435 a.a. ~ 1531 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. RAAPKGTKRDPALNSGVSQKPDPAKTKNRRKRKPSTSDDSDSNFEKIVSKAVTSKKSKGESDDFHMDFDSAVAPRAKSVRAKKPIKYLEESDEDDLF

Localization

Cytoplasmic and Nuclear; nucleoplasm. Note: Generally located in the nucleoplasm.

Specificity

TOP2A - topoisomerase (DNA) II alpha 170kDa

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁ kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for TOP2A Antibody (1E2)

Western Blot: TOP2A Antibody (1E2) [H00007153-M01]

Western Blot: TOP2A Antibody (1E2) [H00007153-M01] - TOP2A monoclonal antibody (M01), clone 1E2 Analysis of TOP2A expression in Hela S3 NE.

Immunocytochemistry/ Immunofluorescence: TOP2A Antibody (1E2) [H00007153-M01]

Immunocytochemistry/Immunofluorescence: TOP2A Antibody (1E2) [H00007153-M01] - Analysis of monoclonal antibody to TOP2A on HeLa cell. Antibody concentration 10 ug/ml.

Immunohistochemistry-Paraffin: TOP2A Antibody (1E2) [H00007153-M01]

Immunohistochemistry-Paraffin: TOP2A Antibody (1E2) [H00007153-M01] - Analysis of monoclonal antibody to TOP2A on formalin-fixed paraffin-embedded human testis. Antibody concentration 3 ug/ml.

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Applications for TOP2A Antibody (1E2)

Application

Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF, IHC-P and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: TOP2A

This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromsome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia.

Long Name

DNA Topoisomerase II alpha

Alternate Names

TOP2, TP2A

Entrez Gene IDs

7153 (Human)

Gene Symbol

TOP2A

OMIM

126430 (Human)

UniProt

NP_001058 (Human)

Additional TOP2A Products

Product Documents for TOP2A Antibody (1E2)

Product Datasheets

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COA

SDS

Product Specific Notices for TOP2A Antibody (1E2)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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