Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # TOP2A/4397R

Format

Azide and BSA Free

Concentration

1 mg/ml

View all TOP2A Antibodies »

Product Specifications

Immunogen

Synthetic peptide corresponding to TOP2A residues within aa1431-1531 of TOP2A (Uniprot: P11388 )

Localization

Nuclear

Marker

Proliferation & Drug-Resistance Marker

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

170 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07735).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for TOP2A Antibody (TOP2A/4397R) - Azide and BSA Free

Immunohistochemistry-Paraffin: TOP2A Antibody (TOP2A/4397R) - Azide and BSA Free [NBP3-08756]

Immunohistochemistry-Paraffin: TOP2A Antibody (TOP2A/4397R) - Azide and BSA Free [NBP3-08756]

Immunohistochemistry-Paraffin: TOP2A Antibody (TOP2A/4397R) - Azide and BSA Free [NBP3-08756] - Formalin-fixed, paraffin-embedded human tonsil stained with TOP2A Recombinant Rabbit Monoclonal Antibody (TOP2A/4397R).
Immunohistochemistry-Paraffin: TOP2A Antibody (TOP2A/4397R) - Azide and BSA Free [NBP3-08756]

Immunohistochemistry-Paraffin: TOP2A Antibody (TOP2A/4397R) - Azide and BSA Free [NBP3-08756]

Immunohistochemistry-Paraffin: TOP2A Antibody (TOP2A/4397R) - Azide and BSA Free [NBP3-08756] - Formalin-fixed, paraffin-embedded human lymph node stained with TOP2A Recombinant Rabbit Monoclonal Antibody (TOP2A/4397R).
TOP2A Antibody (TOP2A/4397R) - Azide and BSA Free

TOP2A Antibody (TOP2A/4397R)

Western blot analysis of Jurkat cell lysate using TOP2A antibody (TOP2A/4397R).
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Applications for TOP2A Antibody (TOP2A/4397R) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry Formalin-fixed: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: TOP2A

Topo II Alpha encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromsome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia.

Long Name

DNA Topoisomerase II alpha

Alternate Names

TOP2, TP2A

Gene Symbol

TOP2A

Additional TOP2A Products

Product Documents for TOP2A Antibody (TOP2A/4397R) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for TOP2A Antibody (TOP2A/4397R) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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