TOP2A Antibody (TOP2A/6570R)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07736
Recombinant Monoclonal Antibody
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # TOP2A/6570R
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Recombinant human TOP2A fragment (aa1352-1493) (exact sequence is proprietary) (Uniprot: P11388 )
Localization
Nuclear
Marker
Proliferation & Drug-Resistance Marker
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
170 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08757)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Scientific Data Images for TOP2A Antibody (TOP2A/6570R)
Western Blot: TOP2A Antibody (TOP2A/6570R) [NBP3-07736]
Western Blot: TOP2A Antibody (TOP2A/6570R) [NBP3-07736] - Western Blot Analysis of HeLa and Jurkat cell lysates using TOP2A antibody (TOP2A/6570R).Immunohistochemistry-Paraffin: TOP2A Antibody (TOP2A/6570R) [NBP3-07736]
Immunohistochemistry-Paraffin: TOP2A Antibody (TOP2A/6570R) [NBP3-07736] - Formalin-fixed, paraffin-embedded human lymph node stained with TOP2A Recombinant Rabbit Monoclonal Antibody (TOP2A/6570R).Immunohistochemistry-Paraffin: TOP2A Antibody (TOP2A/6570R) [NBP3-07736]
Immunohistochemistry-Paraffin: TOP2A Antibody (TOP2A/6570R) [NBP3-07736] - Formalin-fixed, paraffin-embedded human tonsil stained with TOP2A Recombinant Rabbit Monoclonal Antibody (TOP2A/6570R).Applications for TOP2A Antibody (TOP2A/6570R)
Application
Recommended Usage
Immunohistochemistry-Paraffin
1-2 ug/ml
Western Blot
2-4 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: TOP2A
Additional TOP2A Products
Product Documents for TOP2A Antibody (TOP2A/6570R)
Product Specific Notices for TOP2A Antibody (TOP2A/6570R)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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