Topoisomerase I Antibody (6D8)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26278

Recombinant monoclonal antibody expressed in HEK293F cells
Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 6D8

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Topoisomerase I Antibodies »

Product Summary for Topoisomerase I Antibody (6D8)

Immunogen

A synthesized peptide derived from Human Topoisomerase I [UniProt P11387]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Topoisomerase I Antibody (6D8)

Topoisomerase I Antibody (6D8)

Immunoprecipitation: Topoisomerase I Antibody (6D8) [NBP3-26278] -

Immunoprecipitation: Topoisomerase I Antibody (6D8) [NBP3-26278] - Immunoprecipitating Topoisomerase I in K562 whole cell lysate.
Lane 1: Rabbit control IgG instead of NBP3-26278 in K562 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000).
Lane 2: NBP3-26278 (2ug) + K562 whole cell lysate (500ug).
Lane 3: K562 whole cell lysate (10ug).
Topoisomerase I Antibody (6D8)

Immunohistochemistry: Topoisomerase I Antibody (6D8) [NBP3-26278] -

Immunohistochemistry: Topoisomerase I Antibody (6D8) [NBP3-26278] - Image of Topoisomerase I Antibody (6D8) diluted at 1:100 and staining in paraffin-embedded human small intestine tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Topoisomerase I Antibody (6D8)

Immunohistochemistry: Topoisomerase I Antibody (6D8) [NBP3-26278] -

Immunohistochemistry: Topoisomerase I Antibody (6D8) [NBP3-26278] - Image of Topoisomerase I Antibody (6D8) diluted at 1:100 and staining in paraffin-embedded human small intestine tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
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Applications for Topoisomerase I Antibody (6D8)

Application
Recommended Usage

Flow Cytometry

1:20-1:200

Immunohistochemistry

1:50-1:200

Immunoprecipitation

1:200-1:1000

Western Blot

1:500-1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl and 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: Topoisomerase I

Topoisomerases are nuclear enzymes involved in a variety of cellular activities such as chromosome condensation, DNA replication, transcription, recombination and segregation at mitosis. Human topoisomerase I is a 100kD protein capable of relaxing positively and negatively supercoiled DNA by performing a transient single stranded nick which is then religated at the end of the reaction. It has been shown that the enzyme is located in regions of the genome that are undergoing active RNA synthesis, where it probably reduces superhelical stresses in the DNA, enabling RNA polymerase to function properly. Both DNA topoisomerases I and II have been found to be targets of autoantibodies in the sera of patients with certain autoimmune diseases such as systemic lupus erythematosus and also of some anti tumor drugs and antibiotics. Elevated levels of DNA topoisomerase I, detected by transfer assays, have been demonstrated in colorectal tumors compared with normal colon mucosa as a result of increased transcription or mRNA stability.

Long Name

DNA topoisomerase 1

Alternate Names

DNA topoisomerase I, EC 5.6.2.1, TOP1

Gene Symbol

TOP1

Additional Topoisomerase I Products

Product Documents for Topoisomerase I Antibody (6D8)

Product Datasheets

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Product Specific Notices for Topoisomerase I Antibody (6D8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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