TOR/mTOR [p Ser2481] Antibody (JE59-62)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32779
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # JE59-62
Concentration
1 mg/ml
Product Specifications
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding Ser2481 of human TOR/mTOR. (Uniprot: P42345)
Modification
p Ser 2481
Localization
Endoplasmic reticulum membrane, Golgi apparatus membrane, Mitochondrion outer membrane, Lysosome, Cytoplasm, Nucleus, Microsome membrane, Lysosome membrane, Cytoplasmic vesicle, phagosome.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
289 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for TOR/mTOR [p Ser2481] Antibody (JE59-62)
![TOR/mTOR [p Ser2481] Antibody (JE59-62)](https://resources.bio-techne.com/images/products/nbp3-32779_rabbit-tor-mtor-p-ser2481-mab-je59-62-288202412461671.jpg "Western Blot: TOR/mTOR [p Ser2481] Antibody (JE59-62) [NBP3-32779]")
Western Blot: TOR/mTOR [p Ser2481] Antibody (JE59-62) [NBP3-32779]
Western Blot: TOR/mTOR [p Ser2481] Antibody (JE59-62) [NBP3-32779] - Western blot analysis of TOR/mTOR on different lysates with Rabbit anti-TOR/mTOR antibody (NBP3-32779) at 1/5,000 dilution.Lane 1: HeLa whole cell lysate
Lane 2: HeLa starved overnight then treated with 200nM PMA for 4 hours whole cell lysate
Lysates/proteins at 20 ug/Lane.
Predicted band size: 289 kDa
Observed band size: 289 kDa
Exposure time: 5 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32779) at 1/5,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.
Applications for TOR/mTOR [p Ser2481] Antibody (JE59-62)
Application
Recommended Usage
Western Blot
1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: TOR
Long Name
Mammalian Target of Rapamycin
Alternate Names
FRAP1, FRAP2, MTOR, RAFT1
Gene Symbol
MTOR
Additional TOR Products
Product Documents for TOR/mTOR [p Ser2481] Antibody (JE59-62)
Product Specific Notices for TOR/mTOR [p Ser2481] Antibody (JE59-62)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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