Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI2A2

Concentration

0.73 mg/ml

View all TPMT Antibodies »

Product Summary for TPMT Antibody (OTI2A2)

Immunogen

Full length human recombinant protein of human TPMT (NP_000358) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

28.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for TPMT Antibody (OTI2A2)

Western Blot: TPMT Antibody (OTI2A2) [NBP2-02356]

Western Blot: TPMT Antibody (OTI2A2) [NBP2-02356]

Western Blot: TPMT Antibody (2A2) [NBP2-02356] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TPMT (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TPMT.
Immunocytochemistry/ Immunofluorescence: TPMT Antibody (OTI2A2) [NBP2-02356]

Immunocytochemistry/ Immunofluorescence: TPMT Antibody (OTI2A2) [NBP2-02356]

Immunocytochemistry/Immunofluorescence: TPMT Antibody (2A2) [NBP2-02356] - Immunofluorescent staining of HepG2 cells using anti-TPMT mouse monoclonal antibody.
Immunohistochemistry-Paraffin: TPMT Antibody (2A2) [NBP2-02356] Staining of paraffin-embedded liver tissue using anti-TPMT mouse monoclonal antibody.
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Applications for TPMT Antibody (OTI2A2)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50

Immunohistochemistry-Paraffin

1:50

Western Blot

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.73 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: TPMT

This gene encodes the enzyme that metabolizes thiopurine drugs via S-adenosyl-L-methionine as the S-methyl donor and S-adenosyl-L-homocysteine as a byproduct. Thiopurine drugs such as 6-mercaptopurine are used as chemotherapeutic agents. Genetic polymorphisms that affect this enzymatic activity are correlated with variations in sensitivity and toxicity to such drugs within individuals. A pseudogene for this locus is located on chromosome 18q.

Long Name

Thiopurine S-methyltransferase

Alternate Names

EC 2.1.1.67

Entrez Gene IDs

7172 (Human)

Gene Symbol

TPMT

Additional TPMT Products

Product Documents for TPMT Antibody (OTI2A2)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for TPMT Antibody (OTI2A2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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