Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI2A2

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all TPMT Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human TPMT (NP_000358) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

28.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for TPMT Antibody (OTI2A2) - Azide and BSA Free

Western Blot: TPMT Antibody (OTI2A2)Azide and BSA Free [NBP2-74570]

Western Blot: TPMT Antibody (OTI2A2)Azide and BSA Free [NBP2-74570]

Western Blot: TPMT Antibody (OTI2A2) - Azide and BSA Free [NBP2-74570] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TPMT (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TPMT.
Immunocytochemistry/ Immunofluorescence: TPMT Antibody (OTI2A2) - Azide and BSA Free [NBP2-74570]

Immunocytochemistry/ Immunofluorescence: TPMT Antibody (OTI2A2) - Azide and BSA Free [NBP2-74570]

Immunocytochemistry/Immunofluorescence: TPMT Antibody (OTI2A2) - Azide and BSA Free [NBP2-74570] - Staining of HepG2 cells using anti-TPMT mouse monoclonal antibody.
Immunohistochemistry: TPMT Antibody (OTI2A2) - Azide and BSA Free [NBP2-74570]

Immunohistochemistry: TPMT Antibody (OTI2A2) - Azide and BSA Free [NBP2-74570]

Immunohistochemistry: TPMT Antibody (OTI2A2) - Azide and BSA Free [NBP2-74570] - Staining of paraffin-embedded colon tissue using anti-TPMT mouse monoclonal antibody.
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Applications for TPMT Antibody (OTI2A2) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50

Western Blot

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: TPMT

TPMT encodes the enzyme that metabolizes thiopurine drugs via S-adenosyl-L-methionine as the S-methyl donor and S-adenosyl-L-homocysteine as a byproduct. Thiopurine drugs such as 6-mercaptopurine are used as chemotherapeutic agents. Genetic polymorphisms that affect this enzymatic activity are correlated with variations in sensitivity and toxicity to such drugs within individuals. A pseudogene for this locus is located on chromosome 18q.

Long Name

Thiopurine S-methyltransferase

Alternate Names

EC 2.1.1.67

Gene Symbol

TPMT

Additional TPMT Products

Product Documents for TPMT Antibody (OTI2A2) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for TPMT Antibody (OTI2A2) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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