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TPR Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-2867

Novus Biologicals, part of Bio-Techne
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NB100-2867

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 2300 and the C-terminus (residue 2349) of human Translocated Promoter Region Protein using the numbering given in entry NP_003283.1 (GeneID 7175).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TPR Antibody

Immunocytochemistry: TPR Antibody [NB100-2867]

Immunocytochemistry: TPR Antibody [NB100-2867]

NBF-fixed asynchronous HeLa cells. Affinity purified rabbit anti-TPR used at a dilution of 1:250. Detection: anti-rabbit IgG-FITC conjugated used at a dilution of 1:100.
Immunohistochemistry-Paraffin: TPR Antibody [NB100-2867]

Immunohistochemistry-Paraffin: TPR Antibody [NB100-2867]

Immunohistochemistry-Paraffin: TPR Antibody [NB100-2867] - Human testis. Antibody: Affinity purified rabbit anti- TPR used at a dilution of 1:100 (2ug/ml). Detection: DAB
Immunoprecipitation: TPR Antibody [NB100-2867]

Immunoprecipitation: TPR Antibody [NB100-2867]

Immunoprecipitation: TPR Antibody [NB100-2867] - Detection of Human TPR on HeLa whole cell lysate using NB100-2867. TPR was also immunoprecipitated by rabbit anti-TPR antibodies NB100-2864, NB100-2865 and NB100-2866.

Applications for TPR Antibody

Application
Recommended Usage

Immunohistochemistry

1:50 to 1:500

Immunohistochemistry-Paraffin

1:50 to 1:500

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
For IHC: Epitope exposure is recommended. Epitope exposure with citrate buffer will enhance staining. For ICC/IF: Permeabilization with Triton-X 100 is recommended.

Reviewed Applications

Read 1 review rated 3 using NB100-2867 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-buffered Saline, 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: TPR

This gene encodes a large coiled-coil protein that forms intranuclear filaments attached to the inner surface of nuclear pore complexes (NPCs). The protein directly interacts with several components of the NPC. It is required for the nuclear export of mRNAs and some proteins. Oncogenic fusions of the 5' end of this gene with several different kinase genes occur in some neoplasias.

Alternate Names

nuclear pore complex-associated protein TPR, nucleoprotein TPR, translocated promoter region (to activated MET oncogene), tumor potentiating region

Entrez Gene IDs

7175 (Human)

Gene Symbol

TPR

UniProt

Additional TPR Products

Product Documents for TPR Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for TPR Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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