TPT1/TCTP Antibody (10A9)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26272
Recombinant monoclonal antibody expressed in HEK293F cells
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 10A9
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for TPT1/TCTP Antibody (10A9)
Immunogen
A synthesized peptide derived from Human TPT1/TCTP [UniProt P13693]
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for TPT1/TCTP Antibody (10A9)
Immunoprecipitation: TPT1/TCTP Antibody (10A9) [NBP3-26272] -
Immunoprecipitation: TPT1/TCTP Antibody (10A9) [NBP3-26272] - Immunoprecipitating TPT1/TCTP in Hela whole cell lysate.Lane 1: Rabbit control IgG instead of NBP3-26272 in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000).
Lane 2: NBP3-26272 (2ug) + Hela whole cell lysate (500ug).
Lane 3: Hela whole cell lysate (10ug).
Immunohistochemistry: TPT1/TCTP Antibody (10A9) [NBP3-26272] -
Immunohistochemistry: TPT1/TCTP Antibody (10A9) [NBP3-26272] - Image of TPT1/TCTP Antibody (10A9) diluted at 1:100 and staining in paraffin-embedded human prostate tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Western Blot: TPT1/TCTP Antibody (10A9) [NBP3-26272] -
Western Blot: TPT1/TCTP Antibody (10A9) [NBP3-26272] - Positive Western Blot detected in: Hela whole cell lysate, Jurkat whole cell lysate, MCF-7 whole cell lysate, RAW264.7 whole cell lysate, HepG2 whole cell lysate, PC-3 whole cell lysate, Raji whole cell lysate, 293 whole cell lysate, Mouse liver tissue.All lanes: TPT1/TCTP Antibody at 1: 2000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 20, 16 kDa
Observed band size: 25 kDa
Applications for TPT1/TCTP Antibody (10A9)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:20-1:200
Immunohistochemistry
1:50-1:200
Immunoprecipitation
1:200-1:1000
Western Blot
1:500-1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, pH 7.4, 150mM NaCl and 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Background: TPT1/TCTP
Long Name
Translationally-controlled Tumor Protein 1
Alternate Names
Fortilin, HRF, p02, TCTP
Gene Symbol
TPT1
Additional TPT1/TCTP Products
Product Documents for TPT1/TCTP Antibody (10A9)
Product Specific Notices for TPT1/TCTP Antibody (10A9)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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