TPT1/TCTP Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-38447

Novus Biologicals, part of Bio-Techne
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NBP2-38447
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (97%), Rat (97%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all TPT1/TCTP Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: MIIYRDLISHDEMFSDIYKIREIADGLCLEVEGKMVSRTEGNIDDSLIGGNASAEGPEGEGTESTVITGVDIVMNHHLQETSFTKEAYKKYIKDYM

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TPT1/TCTP Antibody

Western Blot: TPT1/TCTP Antibody [NBP2-38447]

Western Blot: TPT1/TCTP Antibody [NBP2-38447]

Western Blot: TPT1/TCTP Antibody [NBP2-38447] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG. Lane 4: Human Plasma. Lane 5: Human liver tissue. Lane 6: Human tonsil tissue
Immunocytochemistry/ Immunofluorescence: TPT1/TCTP Antibody [NBP2-38447]

Immunocytochemistry/ Immunofluorescence: TPT1/TCTP Antibody [NBP2-38447]

Immunocytochemistry/Immunofluorescence: TPT1/TCTP Antibody [NBP2-38447] - Immunofluorescent staining of human cell line HEK 293 shows localization to cytosol.
Immunohistochemistry-Paraffin: TPT1/TCTP Antibody [NBP2-38447]

Immunohistochemistry-Paraffin: TPT1/TCTP Antibody [NBP2-38447]

Immunohistochemistry-Paraffin: TPT1/TCTP Antibody [NBP2-38447] - Staining of human urinary bladder shows moderate cytoplasmic positivity in urothelial cells.

Applications for TPT1/TCTP Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TPT1/TCTP

Histamine is an inflammatory mediator that is ubiquitously expressed and has a broad range of pharmacologic effects. Specifically, it plays a role in the central nervous, gastrointestinal, respiratory and immune systems. Histamine release is mediated by the stimulation of mast cells and basophils. Histamine-releasing factor (HRF) is a cytokine-like molecule that causes the release of histamine, IL-4 and IL-13 from basophils as well as the secretion of IL-8 and a calcium response in eosinophils. HRF belongs to the translationally controlled tumor protein (TCTP) family and is detected as a protein of 21 kDa in mice and 23 kDa in human. It is expressed in several healthy and tumoral cells, including erythrocytes, hepatocytes, macrophages, platelets, keratinocytes, erythroleukemia cells, gliomas, melanomas, hepatoblastomas and lymphomas, and it is localized in the cytoplasm. HRF plays a pivotal role in allergic diseases, and due to its wide distribution in brain, is thought to be involved in neurodegenerative disorders, such as Alzheimer's disease and Down Syndrome.

Long Name

Translationally-controlled Tumor Protein 1

Alternate Names

Fortilin, HRF, p02, TCTP

Gene Symbol

TPT1

UniProt

P13693

Additional TPT1/TCTP Products

Product Documents for TPT1/TCTP Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for TPT1/TCTP Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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