TRA-1-60(R) Antibody (TRA-1-60)

Novus Biologicals, part of Bio-Techne | Catalog # NB100-730

Novus Biologicals, part of Bio-Techne
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NB100-730
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Key Product Details

Species Reactivity

Validated:

Human, Mouse (Negative)

Cited:

Human, Mouse

Applications

Validated:

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgM Clone # TRA-1-60

Concentration

1 mg/ml

View all TRA-1-60(R) Antibodies »

Product Specifications

Immunogen

Human embryonal carcinoma cell line 2102Ep.

Reactivity Notes

No immunoreactivity is seen with murine EC, EG or ES cells.

Localization

Cell Membrane

Specificity

Specific for the neuraminidase resistant form of the TRA-1-60 antigen that is expressed upon the surface of human teratocarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES). TRA-1-60 reacts with a sialidase-sensitive epitope.

Marker

Embryonic Stem Cell Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgM

Scientific Data Images for TRA-1-60(R) Antibody (TRA-1-60)

Western Blot: TRA-1-60(R) Antibody (TRA-1-60) [NB100-730]

Western Blot: TRA-1-60(R) Antibody (TRA-1-60) [NB100-730]

Western Blot: TRA-1-60 (R) Antibody (TRA-1-60(R)) [NB100-730] - Whole cell protein from human ES cells (lane 1), human NTERA-2 cells (lane 2), mouse embryonic fibroblasts (MEF, lane 3) and mouse V6.5 ES cells (lane 4, NBP1-97433) were separated on a 7.5% gel and transferred to PVDF membrane. The membrane was then incubated with anti-TRA1-60(R) antibody at 2 ug/ml and detected with an anti-mouse IgM secondary antibody using chemiluminescence. Note how this antibody specifically detects the TRA1-60(R) epitope only on human cells.
Immunocytochemistry/ Immunofluorescence: TRA-1-60(R) Antibody (TRA-1-60) [NB100-730]

Immunocytochemistry/ Immunofluorescence: TRA-1-60(R) Antibody (TRA-1-60) [NB100-730]

Immunocytochemistry/Immunofluorescence: TRA-1-60(R) Antibody (TRA-1-60) [NB100-730] - TRA-1-60(R) was detected in immersion fixed ADLF1 (top panel) and FAB2 (bottom panel) induced pluripotent stem cell lines using Mouse Anti-Human TRA-1-60(R) Neuraminidase Resistant Epitope Monoclonal Antibody (Catalog # NB100-730) at 10 ug/mL for 3 hours at room temperature. Cells were stained using an Anti-Mouse IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces.
Flow Cytometry: TRA-1-60(R) Antibody (TRA-1-60) [NB100-730]

Flow Cytometry: TRA-1-60(R) Antibody (TRA-1-60) [NB100-730]

Flow Cytometry: TRA-1-60(R) Antibody (TRA-1-60) [NB100-730] - BG01V human embryonic stem cells were stained with Mouse Anti-Human TRA-1-60 Neuraminidase Resistant Epitope Monoclonal Antibody (Catalog # NB100-730, filled histogram) or isotype control antibody followed by Phycoerythrin-conjugated Anti-Mouse IgM Secondary Antibody (Catalog # F0116).
View 2 more images

Applications for TRA-1-60(R) Antibody (TRA-1-60)

Application
Recommended Usage

ELISA

reported in scientific literature (PMID 30617132)

Flow Cytometry

1:50-1:200

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry

1:50-1:200

Immunohistochemistry-Paraffin

reported in scientific literature (PMID 33234532)

Immunoprecipitation

1:10-1:500

Western Blot

1:100-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgM purified

Formulation

PBS

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TRA-1-60(R)

TRA-1-60 is a cell-surface protein involved in cell differentiation. Cell surface antigens are powerful tools for characterizing the function and expression of developmentally regulated cells during both embryogenesis and oncogenesis. The TRA-1-60 epitope is also found on human embryonic stem (ES) cells and primordial germ cells, and TRA-1-60 serves as a serum marker in patients with germ cell tumors. Investigation into the identity of the TRA-1-60 epitope demonstrated that it is a carbohydrate carried by a cell surface, sialylated, keratan sulfate proteoglycan. Subsequent evidence implicated podocalyxin as a carrier for the TRA-1-60 epitope.

Long Name

Tumor-related Antigen-1-60 [R]

Alternate Names

TRA160(R)

Additional TRA-1-60(R) Products

Product Documents for TRA-1-60(R) Antibody (TRA-1-60)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for TRA-1-60(R) Antibody (TRA-1-60)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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