TRAF-2 Antibody (33A1293) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB100-56715

Novus Biologicals, part of Bio-Techne
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NB100-56715
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 33A1293

Format

BSA Free

Concentration

1.0 mg/ml

View all TRAF-2 Antibodies »

Product Specifications

Immunogen

Anti-TRAF2 monoclonal antibody was raised against a fusion protein corresponding to amino acids 205 to 222 of human TRAF2.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for TRAF-2 Antibody (33A1293) - BSA Free

Western Blot: TRAF-2 Antibody (33A1293)BSA Free [NB100-56715]

Western Blot: TRAF-2 Antibody (33A1293)BSA Free [NB100-56715]

Western Blot: TRAF-2 Antibody (33A1293) [NB100-56715] - Analysis of TRAF2 in HeLa lysate using TRAF2 antibody at 2 ug/ml.
Immunohistochemistry-Paraffin: TRAF-2 Antibody (33A1293) - BSA Free [NB100-56715]

Immunohistochemistry-Paraffin: TRAF-2 Antibody (33A1293) - BSA Free [NB100-56715]

Immunohistochemistry-Paraffin: TRAF-2 Antibody (33A1293) [NB100-56715] - Human transitional cell carcinoma of the urinary bladder stained with TRAF2 antibody at 5 ug/ml. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.

Applications for TRAF-2 Antibody (33A1293) - BSA Free

Application
Recommended Usage

Immunohistochemistry

2:20-2:1000

Immunohistochemistry-Paraffin

1:10-1:500. Use reported in scientific literature (Galen et al (2008))

Western Blot

2 ug/ml
Application Notes
In HeLa, an approx. 57 kDa band is observed.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TRAF-2

Tumor necrosis factor (TNF) induced signaling is mediated through association of TNF receptor (TNFR) with adaptor proteins, such as TNF receptor associated proteins (TRAFs). TRAFs form a family of cytoplasmic adapter proteins that mediate signal transduction from many members of the TNF-receptor superfamily (e.g. RANK, CD30, CD40, etc.) and the interleukin-1 receptor. The carboxy-terminal region of TRAFs is required for self-association and interaction with receptor cytoplasmic domains following ligand-induced oligomerization. Recent molecular cloning studies have lead to identification of six TRAFs (TRAF1-TRAF6). TRAF2 is a 502-amino acid protein. Mutagenic studies suggest that the N-terminal RING finger and two adjacent zinc fingers of TRAF2 are required for NF-kB activation, where as interaction with TNFR is mediated through C-terminus domain. Distinct domains in the N- and -C termini are also required for association with TRAF1 and protein kinase receptor interacting protein (RIP). TRAF2 is involved in cellular resistant to TNF-induced apoptosis. TRAF-2 deficient mice appeared normal at birth, however, they die prematurely, probably due to atrophy of thymus, spleen, muscle mass and lack of TRAF-2's cytoprotective role.

Long Name

TNF Receptor-Associated Factor 2

Alternate Names

TRAF2, TRAP3

Entrez Gene IDs

7186 (Human)

Gene Symbol

TRAF2

UniProt

Q12933

Additional TRAF-2 Products

Product Documents for TRAF-2 Antibody (33A1293) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for TRAF-2 Antibody (33A1293) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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