TRAF-3 Antibody (PSH01-30)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-33042
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # PSH01-30
Concentration
1 mg/ml
Product Summary for TRAF-3 Antibody (PSH01-30)
Immunogen
Recombinant protein within human TRAF-3 301-568 / 568. (Uniprot: Q13114)
Localization
Cytoplasm, Endosome, Mitochondrion.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
64 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for TRAF-3 Antibody (PSH01-30)
Western Blot: TRAF-3 Antibody (PSH01-30) [NBP3-33042]
Western Blot: TRAF-3 Antibody (PSH01-30) [NBP3-33042] - Western blot analysis of TRAF-3 on different lysates with Rabbit anti-TRAF-3 antibody (NBP3-33042) at 1/1,000 dilution.Lane 1: TF-1 cell lysate (20 ug/Lane)
Lane 2: THP-1 cell lysate (20 ug/Lane)
Lane 3: 293T cell lysate (20 ug/Lane)
Lane 4: Jurkat cell lysate (20 ug/Lane)
Lane 5: A549 cell lysate (20 ug/Lane)
Lane 6: RAW264.7 cell lysate (20 ug/Lane)
Lane 7: NIH/3T3 cell lysate (20 ug/Lane)
Lane 8: Mouse brain tissue lysate (40 ug/Lane)
Lane 9: Rat spleen tissue lysate (40 ug/Lane)
Lysates/proteins at 10 ug/Lane.
Predicted band size: 64 kDa
Observed band size: 50 kDa
Exposure time: 1 minute 9 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33042) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.
Applications for TRAF-3 Antibody (PSH01-30)
Application
Recommended Usage
Western Blot
1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH7.4), 0.1% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: TRAF-3
Long Name
TNF Receptor-Associated Factor 3
Alternate Names
CAP1, CD40bp, CRAF1, LAP1, TRAF3
Gene Symbol
TRAF3
Additional TRAF-3 Products
Product Documents for TRAF-3 Antibody (PSH01-30)
Product Specific Notices for TRAF-3 Antibody (PSH01-30)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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