TRAF-6 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-56436

Novus Biologicals, part of Bio-Techne
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NB100-56436
NB100-56436SS
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (98%), Mouse (94%), Porcine (98%), Rat (94%), Rhesus Macaque (98%). Backed by our 100% Guarantee.

Applications

Validated:

Flow Cytometry, Western Blot

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all TRAF-6 Antibodies »

Product Specifications

Immunogen

Anti-TRAF6 polyclonal antibody was raised against a peptide corresponding to amino acids between 410 and 460 of human TRAF6.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: sooty mangabey (Cercocebus atys) (98%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TRAF-6 Antibody

Western Blot: TRAF-6 Antibody [NB100-56436]

Western Blot: TRAF-6 Antibody [NB100-56436]

Western Blot: TRAF-6 Antibody [NB100-56436] - Analysis of TRAF-6 using NB100-56436 in A) human thymus cell lysate and B) human testis cell lysate.

Applications for TRAF-6 Antibody

Application
Recommended Usage

Western Blot

3-5 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

0.1 ml PBS

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TRAF-6

Tumor necrosis factor (TNF) induced signaling is mediated through association of TNF receptor (TNFR) with adaptor proteins, such as TNF receptor associated proteins (TRAFs). TRAFs form a family of cytoplasmic adapter proteins that mediate signal transduction from many members of the TNF-receptor superfamily (e.g. RANK, CD30, CD40, etc.) and the interleukin-1 receptor. The carboxy-terminal region of TRAFs is required for self-association and interaction with receptor cytoplasmic domains following ligand-induced oligomerization. Recent molecular cloning studies have lead to identification of six TRAFs (TRAF1-TRAF6) (1-4). Recently it has been shown that TRANCE/OPGL activates the antiapoptotic serine/threonine kinase Akt/PKB through a signaling complex involving c-Src and TRAF6 (5). Mice deficient in TNF receptor-associated factor 6 (TRAF6) are osteopetrotic with defects in bone remodeling and tooth eruption due to impaired osteoclast function (6). Like TRAF2 and TRAF3, TRAF6 is also essential for perinatal and postnatal survival. These findings establish unexpectedly diverse and critical roles for TRAF6 in perinatal and postnatal survival, bone metabolism, LPS, and cytokine signaling. RANK (TRANCE receptor) interacts with various TRAFs through distinct motifs and activates NF-kB via a novel TRAF6 interaction motif, which then activates NIK, thus leading to NF-kB activation (7). Over-expression of TRAF6 activates JNK, p38, or IKK in the absence of extracellular stimulation.

Long Name

TNF Receptor-Associated Factor 6

Alternate Names

TRAF6

Entrez Gene IDs

7189 (Human); 22034 (Mouse)

Gene Symbol

TRAF6

UniProt

Q9Y4K3

Additional TRAF-6 Products

Product Documents for TRAF-6 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for TRAF-6 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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