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TRAFD1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-81875

Novus Biologicals, part of Bio-Techne
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NBP1-81875
NBP1-81875-25ul

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Summary for TRAFD1 Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: PSSPCVPKLSNSDSQDIQGRNRDSQNGAIAPGHVSVIRPPQNLYPENIVPSFSPGPSGRYGASGRSEGGRNSRVTPAAANYRSRTAKAKPSKQQGAGD

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TRAFD1 Antibody

Western Blot: TRAFD1 Antibody [NBP1-81875]

Western Blot: TRAFD1 Antibody [NBP1-81875]

Western Blot: TRAFD1 Antibody [NBP1-81875] - Analysis in control (vector only transfected HEK293T lysate) and TRAFD1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: TRAFD1 Antibody [NBP1-81875]

Immunocytochemistry/ Immunofluorescence: TRAFD1 Antibody [NBP1-81875]

Immunocytochemistry/Immunofluorescence: TRAFD1 Antibody [NBP1-81875] - Staining of human cell line U-251 MG shows localization to nucleoplasm and cytosol.
Immunohistochemistry-Paraffin: TRAFD1 Antibody [NBP1-81875]

Immunohistochemistry-Paraffin: TRAFD1 Antibody [NBP1-81875]

Immunohistochemistry-Paraffin: TRAFD1 Antibody [NBP1-81875] - Staining in human testis and skeletal muscle tissues using anti-TRAFD1 antibody. Corresponding TRAFD1 RNA-seq data are presented for the same tissues.

Applications for TRAFD1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TRAFD1

The innate immune system confers host defense against viral and microbial infection, and TRAFD1 is a negative feedback regulator that controls excessive immune responses (Sanada et al., 2008 (PubMed 18849341)).(supplied by OMIM)

Alternate Names

FLN29FLN29 gene product, Protein FLN29, TRAF-type zinc finger domain containing 1, TRAF-type zinc finger domain-containing protein 1

Gene Symbol

TRAFD1

Additional TRAFD1 Products

Product Documents for TRAFD1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for TRAFD1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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