TRAILR1/TNFRSF10A Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-25092
Conjugate
Catalog #
Key Product Details
Validated by
Knockout/Knockdown, Independent Antibodies
Species Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Antibody was raised against a peptide corresponding to 19 amino acids near the carboxy terminus of human DR4 protein. The immunogen is located within the last 50 amino acids of DR4. Amino Acid Squence: DSGKFIYLEDGTGSAVSLE
Specificity
Human TRAILR1/TNFRSF10A has only 1 isoform (468aa, 50kD). TRAILR1/TNFRSF10A antibody has no cross reaction to TRAILR2/TNFRSF10B.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for TRAILR1/TNFRSF10A Antibody - BSA Free
Western Blot: TRAILR1/TNFRSF10A AntibodyBSA Free [NBP2-25092]
Western Blot: TRAILR1/TNFRSF10A Antibody [NBP2-25092] - Western Blot Validation in Cell Lines. Loading: 15 ug of cell lysates per lane. Antibodies: DR4 NBP2-25092 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Immunohistochemistry: TRAILR1/TNFRSF10A Antibody - BSA Free [NBP2-25092]
Immunohistochemistry: TRAILR1/TNFRSF10A Antibody [NBP2-25092] - Immunofluorescence Validation of DR4. Immunofluorescent analysis of 4% paraformaldehyde-fixed human spleen tissue labeling DR4 with NBP2-25092 at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody (red) and DAPI staining (blue). Image showing membrane staining on human spleen cells.Western Blot: TRAILR1/TNFRSF10A AntibodyBSA Free [NBP2-25092]
Western Blot: TRAILR1/TNFRSF10A Antibody [NBP2-25092] - KO Validation in HeLa Cells. Loading: 10 ug of HeLa WT cell lysates or TRAILR1/TNFRSF10A KO cell lysates. Antibodies: TRAILR1/TNFRSF10A [NBP2-25092] (1 ug/ml) and beta-actin [NBP1-76692] (1 ug/ml), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Applications for TRAILR1/TNFRSF10A Antibody - BSA Free
Application
Recommended Usage
ELISA
1:100-1:2000
Immunocytochemistry/ Immunofluorescence
20 ug/mL
Immunohistochemistry
10 ug/ml
Immunohistochemistry-Paraffin
10 ug/mL
Western Blot
1 ug/mL
Formulation, Preparation, and Storage
Purification
Peptide affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: TRAILR1/TNFRSF10A
Long Name
TRAIL Receptor 1
Alternate Names
CD261, DR4, TNFRSF10A, TRAIL R1
Gene Symbol
TNFRSF10A
UniProt
Additional TRAILR1/TNFRSF10A Products
Product Documents for TRAILR1/TNFRSF10A Antibody - BSA Free
Product Specific Notices for TRAILR1/TNFRSF10A Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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