TRAILR2/TNFRSF10B Antibody - Azide Free

Western Blot: TRAILR2/TNFRSF10B AntibodyAzide Free [NB100-55744]

Western Blot: TRAILR2/TNFRSF10B Antibody - Azide Free [NB100-55744] - Total protein from HL-60, HepG2, HCT-116 and human placenta was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-TRAIL R2 in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Image using the standard format of this product.

Immunocytochemistry/ Immunofluorescence: TRAILR2/TNFRSF10B Antibody - Azide Free [NB100-55744]

Immunocytochemistry/Immunofluorescence: TRAILR2/TNFRSF10B Antibody - Azide Free [NB100-55744] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-TRAIL R2 (H76) at 5ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective. Image using the standard format of this product.

Immunohistochemistry-Paraffin: TRAILR2/TNFRSF10B Antibody - Azide Free [NB100-55744]

Immunohistochemistry-Paraffin: TRAILR2/TNFRSF10B Antibody - Azide Free [NB100-55744] - Analysis of a formalin fixed paraffin-embedded (FFPE) human brain cerebellum using 1:200 conc. of TRAIL R2/TNFRSF10B antibody on a Bond Rx autostainer (Leica Biosystems). The assay involved 30 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 9.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 15 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Cytoplasmic staining was observed in the Purkinje cell layer. Image using the standard format of this product.