TRANCE/TNFSF11/RANK L Antibody (8A7B9) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-61813

Novus Biologicals, part of Bio-Techne
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NBP2-61813
NBP2-61813-0.025ml
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Key Product Details

Species Reactivity

Validated:

Human, Monkey

Cited:

Mouse

Applications

Validated:

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunohistochemistry-Frozen

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 8A7B9

Format

BSA Free

Concentration

1 mg/ml

View all TRANCE/TNFSF11/RANK L Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human TRANCE/TNFSF11/RANK L (AA: 74-308) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

35.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for TRANCE/TNFSF11/RANK L Antibody (8A7B9) - BSA Free

Western Blot: TRANCE/TNFSF11/RANK L Antibody (8A7B9)BSA Free [NBP2-61813]

Western Blot: TRANCE/TNFSF11/RANK L Antibody (8A7B9)BSA Free [NBP2-61813]

Western Blot: TRANCE/TNFSF11/RANK L Antibody (8A7B9) [NBP2-61813] - Analysis using TNFSF11 mAb against HEK293 (1) and TNFSF11 (AA: 74-308)-hIgGFc transfected HEK293 (2) cell lysate.
Immunocytochemistry/ Immunofluorescence: TRANCE/TNFSF11/RANK L Antibody (8A7B9) - BSA Free [NBP2-61813]

Immunocytochemistry/ Immunofluorescence: TRANCE/TNFSF11/RANK L Antibody (8A7B9) - BSA Free [NBP2-61813]

Immunocytochemistry/Immunofluorescence: TRANCE/TNFSF11/RANK L Antibody (8A7B9) [NBP2-61813] - Analysis of Hela cells using TNFSF11 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.
Immunohistochemistry-Paraffin: TRANCE/TNFSF11/RANK L Antibody (8A7B9) - BSA Free [NBP2-61813]

Immunohistochemistry-Paraffin: TRANCE/TNFSF11/RANK L Antibody (8A7B9) - BSA Free [NBP2-61813]

Immunohistochemistry-Paraffin: TRANCE/TNFSF11/RANK L Antibody (8A7B9) [NBP2-61813] - Analysis of bladder cancer tissues using TNFSF11 mouse mAb with DAB staining.
View 4 more images

Applications for TRANCE/TNFSF11/RANK L Antibody (8A7B9) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Immunocytochemistry/ Immunofluorescence

1:100-1:500

Immunohistochemistry

1:200-1:1000

Immunohistochemistry-Paraffin

1:200-1:1000

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TRANCE/TNFSF11/RANK L

This gene encodes a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. This protein was shown to be a dentritic cell survival factor and is involved in the regulation of T cell-dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss. This protein was shown to activate antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor-associated factor (TRAF) 6, which indicated this protein may have a role in the regulation of cell apoptosis. Targeted disruption of the related gene in mice led to severe osteopetrosis and a lack of osteoclasts. The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy. Two alternatively spliced transcript variants have been found.

Long Name

TNF-related Activation-induced Cytokine

Alternate Names

CD254, ODF, OPGL, RANK L, RANKL, TNFSF11

Gene Symbol

TNFSF11

Additional TRANCE/TNFSF11/RANK L Products

Product Documents for TRANCE/TNFSF11/RANK L Antibody (8A7B9) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for TRANCE/TNFSF11/RANK L Antibody (8A7B9) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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