TRANCE/TNFSF11/RANK L Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-31140

Novus Biologicals, part of Bio-Techne
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NBP1-31140
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Predicted:

Bovine (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Microarray, Western Blot

Cited:

Microarray

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all TRANCE/TNFSF11/RANK L Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human TRANCE/TNFSF11/RANK L. The exact sequence is proprietary.

Localization

Isoform 1: Cell membrane, Single-pass type II membrane protein, Isoform 3: Cell membrane, Single-pass type II membrane protein, Isoform 2: Cytoplasm, Tumor necrosis factor ligand superfamily member 11, soluble form: Secreted

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

35 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for TRANCE/TNFSF11/RANK L Antibody

Western Blot: TRANCE/TNFSF11/RANK L Antibody [NBP1-31140]

Western Blot: TRANCE/TNFSF11/RANK L Antibody [NBP1-31140]

Western Blot: TRANCE/TNFSF11/RANK L Antibody [NBP1-31140] - A. 50 ug rat lung extract 10 % SDS-PAGE RANKL antibody [C2C3], C-term dilution: 1:1000
Immunohistochemistry-Paraffin: TRANCE/TNFSF11/RANK L Antibody [NBP1-31140]

Immunohistochemistry-Paraffin: TRANCE/TNFSF11/RANK L Antibody [NBP1-31140]

Immunohistochemistry-Paraffin: TRANCE/TNFSF11/RANK L Antibody [NBP1-31140] - Paraffin-embedded human breast carcinoma. RANKL antibody [C2C3], C-term diluted at 1:500.
Western Blot: TRANCE/TNFSF11/RANK L Antibody [NBP1-31140]

Western Blot: TRANCE/TNFSF11/RANK L Antibody [NBP1-31140]

Western Blot: TRANCE/TNFSF11/RANK L Antibody [NBP1-31140] - Sample (30 ug of whole cell lysate) A: Hela 10% SDS PAGE RANKL (CD254) antibody diluted at 1:1000

Applications for TRANCE/TNFSF11/RANK L Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000
Application Notes
Use in microarray reported in scientific literature (PMID: 28955722).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: TRANCE/TNFSF11/RANK L

This gene encodes a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. This protein was shown to be a dentritic cell survival factor and is involved in the regulation of T cell-dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss. This protein was shown to activate antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor-associated factor (TRAF) 6, which indicated this protein may have a role in the regulation of cell apoptosis. Targeted disruption of the related gene in mice led to severe osteopetrosis and a lack of osteoclasts. The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy. Two alternatively spliced transcript variants have been found.

Long Name

TNF-related Activation-induced Cytokine

Alternate Names

CD254, ODF, OPGL, RANK L, RANKL, TNFSF11

Gene Symbol

TNFSF11

Additional TRANCE/TNFSF11/RANK L Products

Product Documents for TRANCE/TNFSF11/RANK L Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for TRANCE/TNFSF11/RANK L Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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