Transcobalamin II Antibody (2F4)
Novus Biologicals, part of Bio-Techne | Catalog # H00006948-M01
Key Product Details
Species Reactivity
Human
Applications
ELISA, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 2F4
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
TCN2 (AAH11239, 1 a.a. ~ 400 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MRHLGAFLFLLGVLGALTEMCEIPEMDSHLVEKSGQHLLPWMDRLSLEHLNPSIYVGLRLSSLQAGTKEDLYLHSLKLGYQQCLLGSAFSEDDGDCQGKPSMGQLALYLLALRANWHDHKGHPHTSYYQYGLGILALCLHQKRVHDSVVDKLLYAVEPFHQGHHSVDTAAMAGLAFTCLKRSNFNPGRRQRITMAIRTVREEILKAQTPEGHFGNVYSTPLALQFLMTSPMRGAELGTACLKARVALLASLQDGAFQNALMISQLLPVLNHKTYIDLIFPDCLAPRVMLEPAAETIPQTQEIISVTLQVLSLLPPYRQSISVLAGSTVEDVLKKAHELGGFTYETQASLSGPYLTSVMGKAAGEREFWQLLRDPNTPLLQGIADYRPKDGETIELRLVSW
Specificity
TCN2 - transcobalamin II; macrocytic anemia (2F4)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for Transcobalamin II Antibody (2F4)
![Western Blot: Transcobalamin II Antibody (2F4) [H00006948-M01]](https://resources.bio-techne.com/images/products/Transcobalamin-II-Antibody-2F4-Western-Blot-H00006948-M01-img0002.jpg "Western Blot: Transcobalamin II Antibody (2F4) [H00006948-M01]")
Western Blot: Transcobalamin II Antibody (2F4) [H00006948-M01]
Western Blot: Transcobalamin II Antibody (2F4) [H00006948-M01] - Analysis of TCN2 expression in transfected 293T cell line by TCN2 monoclonal antibody (M01), clone 2F4.Lane 1: TCN2 transfected lysate (Predicted MW: 47.5 KDa).Lane 2: Non-transfected lysate.
Sandwich ELISA: Transcobalamin II Antibody (2F4) [H00006948-M01] - Detection limit for recombinant GST tagged TCN2 is 1 ng/ml as a capture antibody.
Applications for Transcobalamin II Antibody (2F4)
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Sandwich ELISA
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Transcobalamin II
Alternate Names
TC-2, TCII, TCN2, Vitamin B12-binding Protein 2
Entrez Gene IDs
6948 (Human)
Gene Symbol
TCN2
UniProt
Additional Transcobalamin II Products
Product Documents for Transcobalamin II Antibody (2F4)
Product Specific Notices for Transcobalamin II Antibody (2F4)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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