Transcription factor E3 Antibody (CL12822) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-44162
![Immunohistochemistry-Paraffin: Transcription factor E3 Antibody [NBP3-44162] Transcription factor E3 Antibody (CL12822) - Azide and BSA Free Immunohistochemistry-Paraffin: Transcription factor E3 Antibody [NBP3-44162]](https://resources.bio-techne.com/images/products/nbp3-44162_mouse-transcr-factor-e3-mab-cl12822-azide-bsa-free-282202512295623.jpg)
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # CL12822
Format
Azide and BSA Free
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
This antibody was generated using a recombinant protein sequence of P19532, with the exact immunogen sequence remaining proprietary.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Transcription factor E3 Antibody (CL12822) - Azide and BSA Free
Immunohistochemistry-Paraffin: Transcription factor E3 Antibody [NBP3-44162]
Staining of human kidney shows weak to moderate nuclear positivity in cells in tubules.Immunohistochemistry-Paraffin: Transcription factor E3 Antibody [NBP3-44162]
Staining of human kidney cancer shows strong nuclear positivity in tumor cells.Immunohistochemistry-Paraffin: Transcription factor E3 Antibody [NBP3-44162]
Staining of human ovarian cancer (clear cell carcinoma) shows moderate nuclear positivity in tumor cells.Applications for Transcription factor E3 Antibody (CL12822) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
2-10 ug/ml
Immunohistochemistry-Paraffin
1:1000 - 1:2500
Western Blot
1 ug/ml
Application Notes
For use with IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/ Immunofluorescence/IF, Fixation/Permeabilization: PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Protein A purified
Reconstitution
Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.
Formulation
Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
Format
Azide and BSA Free
Concentration
LYOPH mg/ml
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Transcription factor E3
Alternate Names
BHLHE33, bHLHe33transcription factor E3, Class E basic helix-loop-helix protein 33, RCCP2, TFEA, transcription factor binding to IGHM enhancer 3, transcription factor E family, member A, Transcription factor for IgH enhancer
Gene Symbol
TFE3
Additional Transcription factor E3 Products
Product Documents for Transcription factor E3 Antibody (CL12822) - Azide and BSA Free
Product Specific Notices for Transcription factor E3 Antibody (CL12822) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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