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Transglutaminase 2/TGM2 Antibody (TGM2/6943R)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13790

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
Catalog #
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NBP3-13790-100ug
NBP3-13790-20ug

Key Product Details

Species Reactivity

Validated:

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # TGM2/6943R

Concentration

0.2 mg/ml

Product Summary for Transglutaminase 2/TGM2 Antibody (TGM2/6943R)

Immunogen

Recombinant full-length human Transglutaminase 2/TGM2 protein (Uniprot: P21980)

Localization

Cytoplasm, Cell surface

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14193)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C. Non-hazardous.

Theoretical molecular weight: 77-85kDa

Scientific Data Images for Transglutaminase 2/TGM2 Antibody (TGM2/6943R)

Immunohistochemistry-Paraffin: Transglutaminase 2/TGM2 Antibody (TGM2/6943R) [NBP3-13790]

Immunohistochemistry-Paraffin: Transglutaminase 2/TGM2 Antibody (TGM2/6943R) [NBP3-13790]

Immunohistochemistry-Paraffin: Transglutaminase 2/TGM2 Antibody (TGM2/6943R) [NBP3-13790] - Formalin-fixed, paraffin-embedded human placenta stained with Transglutaminase 2/TGM2 antibody (TGM2/6943R). Inset: PBS instead of primary antibody; secondary only negative control.
Immunohistochemistry-Paraffin: Transglutaminase 2/TGM2 Antibody (TGM2/6943R) [NBP3-13790]

Immunohistochemistry-Paraffin: Transglutaminase 2/TGM2 Antibody (TGM2/6943R) [NBP3-13790]

Immunohistochemistry-Paraffin: Transglutaminase 2/TGM2 Antibody (TGM2/6943R) [NBP3-13790] - Formalin-fixed, paraffin-embedded human ovarian carcinoma stained with Transglutaminase 2/TGM2 antibody (TGM2/6943R). HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.

Applications for Transglutaminase 2/TGM2 Antibody (TGM2/6943R)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Transglutaminase 2/TGM2

TGase II is implicated in programmed cell death, signal transduction, drugresistance, cell growth, endocytosis, insulin secretion, cell adhesion, cataract formation, and wound healing.

Alternate Names

TGC, TGM2, Transglutaminase 2, tTG

Gene Symbol

TGM2

UniProt

Additional Transglutaminase 2/TGM2 Products

Product Documents for Transglutaminase 2/TGM2 Antibody (TGM2/6943R)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Transglutaminase 2/TGM2 Antibody (TGM2/6943R)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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