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Transglutaminase 3/TGM3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-86950

Novus Biologicals, part of Bio-Techne
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NBP1-86950

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: GSDQERQVFQKALGKLKPNTPFAATSSMGLETEEQEPSIIGKLKVAGMLAVGKEVNLVLLLKNLSRDTKTVTVNMTAWTIIYNGTLVHEVWKDSATMSLDPEEEAEHPIKISYAQYEKYLKSDNMI

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Transglutaminase 3/TGM3 Antibody

Western Blot: Transglutaminase 3/TGM3 Antibody [NBP1-86950]

Western Blot: Transglutaminase 3/TGM3 Antibody [NBP1-86950]

Western Blot: Transglutaminase 3/TGM3 Antibody [NBP1-86950] - Analysis in human cell line EFO-21.
Immunohistochemistry-Paraffin: Transglutaminase 3/TGM3 Antibody [NBP1-86950]

Immunohistochemistry-Paraffin: Transglutaminase 3/TGM3 Antibody [NBP1-86950]

Immunohistochemistry-Paraffin: Transglutaminase 3/TGM3 Antibody [NBP1-86950] - Staining of human esophagus shows high expression.
Immunohistochemistry-Paraffin: Transglutaminase 3/TGM3 Antibody [NBP1-86950]

Immunohistochemistry-Paraffin: Transglutaminase 3/TGM3 Antibody [NBP1-86950]

Immunohistochemistry-Paraffin: Transglutaminase 3/TGM3 Antibody [NBP1-86950] - Staining of human lymph node shows no positivity in germinal center cells and non-germinal center cells as expected.

Applications for Transglutaminase 3/TGM3 Antibody

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Transglutaminase 3/TGM3

Transglutaminases are enzymes that catalyze the crosslinking of proteins by epsilon gamma glutamyl lysine isopeptide bonds. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium dependent. Transglutaminase 3 consists of two polypeptide chains activated from a single precursor protein by proteolysis. Transglutaminase 3 is involved the later stages of cell envelope formation in the epidermis and hair follicle.

Alternate Names

TG3, TgaseE, TGM3

Gene Symbol

TGM3

Additional Transglutaminase 3/TGM3 Products

Product Documents for Transglutaminase 3/TGM3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Transglutaminase 3/TGM3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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