TRAP1 Antibody (1L8C1)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16303

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16303-100ul
NBP3-16303-20ul
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1L8C1

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all TRAP1 Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human TRAP1 (Q12931). MARELRALLLWGRRLRPLLRAPALAAVPGGKPILCPRRTTAQLGPRRNPAWSLQAGRLFSTQTAEDKEEPLHSIISSTESVQGSTSKHEFQAETKKLLDI

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TRAP1 Antibody (1L8C1)

Western Blot: TRAP1 Antibody (1L8C1) [NBP3-16303]

Western Blot: TRAP1 Antibody (1L8C1) [NBP3-16303]

Western Blot: TRAP1 Antibody (1L8C1) [NBP3-16303] - Western blot analysis of extracts of various cell lines, using TRAP1 Rabbit mAb (NBP3-16303) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
Immunoprecipitation: TRAP1 Antibody (1L8C1) [NBP3-16303]

Immunoprecipitation: TRAP1 Antibody (1L8C1) [NBP3-16303]

Immunoprecipitation: TRAP1 Antibody (1L8C1) [NBP3-16303] - Immunoprecipitation analysis of 300ug extracts of HCT116 cells using 3ug TRAP1 antibody (NBP3-16303). Western blot was performed from the immunoprecipitate using TRAP1 antibody (NBP3-16303) at a dilition of 1:500.

Applications for TRAP1 Antibody (1L8C1)

Application
Recommended Usage

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: TRAP1

The 90 kDa heat shock protein (hsp90) family of molecular chaperones is a highly conserved family of proteins that play an important physiological role. Hsp90 is involved in numerous cellular processes but is best known for its association with signal transduction machinery. A recently cloned homolog of hsp90 is TRAP1. Like hsp90, TRAP1 is found to be associated with numerous proteins involved in diverse actions. Immunofluorescence data has shown TRAP1 to be localized in the mitochondria of mammalian cells. This observation and the fact that TRAP1 is shown to have a mitochondrial targeting presequence strongly implicates TRAP1 as a mitochondrial matrix protein. Immunofluorescence staining of TRAP1 in PC-3-M cells with this antibody produces a pattern consistent with mitochondrial staining. Immunoprecipitation of TRAP1 using this antibody fails to co-precipitate p23, Hop, or CyP40 suggesting TRAP1®s inability to associate with these co-chaperones.

Alternate Names

HSP 75, HSP75TNFR-associated protein 1, HSP90Lheat shock protein 75 kDa, mitochondrial, TNF receptor-associated protein 1, TRAP-1, tumor necrosis factor type 1 receptor associated protein, Tumor necrosis factor type 1 receptor-associated protein

Gene Symbol

TRAP1

Additional TRAP1 Products

Product Documents for TRAP1 Antibody (1L8C1)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for TRAP1 Antibody (1L8C1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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