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Trappin-2/Elafin/Skalp Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-85690

Novus Biologicals, part of Bio-Techne
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NBP1-85690
NBP1-85690-25ul

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: KGQDTVKGRVPFNGQDPVKGQVSVKGQDKVKAQEPVKGPVSTKPGSCPIILIRCAMLNPPNRCLKDTDCPGIKKCCEGSCGMA

Reactivity Notes

Reactivity reported in scientific literature (PMID: 23631827)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Trappin-2/Elafin/Skalp Antibody

Immunohistochemistry-Paraffin: Trappin-2/Elafin/Skalp Antibody [NBP1-85690]

Immunohistochemistry-Paraffin: Trappin-2/Elafin/Skalp Antibody [NBP1-85690]

Immunohistochemistry-Paraffin: Trappin-2/Elafin/Skalp Antibody [NBP1-85690] - Staining in human esophagus and liver tissues using NBP1-85690 antibody. Corresponding PI3 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: Trappin-2/Elafin/Skalp Antibody [NBP1-85690]

Immunohistochemistry-Paraffin: Trappin-2/Elafin/Skalp Antibody [NBP1-85690]

Immunohistochemistry-Paraffin: Trappin-2/Elafin/Skalp Antibody [NBP1-85690] - Staining of human endometrium shows no positivity in glandular cells as expected.
Immunohistochemistry-Paraffin: Trappin-2/Elafin/Skalp Antibody [NBP1-85690]

Immunohistochemistry-Paraffin: Trappin-2/Elafin/Skalp Antibody [NBP1-85690]

Immunohistochemistry-Paraffin: Trappin-2/Elafin/Skalp Antibody [NBP1-85690] - Staining of human esophagus shows moderate membranous positivity in squamous epithelial cells.

Applications for Trappin-2/Elafin/Skalp Antibody

Application
Recommended Usage

Immunohistochemistry

1:2500 - 1:5000

Immunohistochemistry-Paraffin

1:2500 - 1:5000

Western Blot

0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. WB and IHC reactivity reported in (PMID: 27444067).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Trappin-2/Elafin

The Skalp gene encodes an elastase-specific inhibitor that functions as an antimicrobial peptide against Gram-positive and Gram-negative bacteria. The protein contains a WAP-type four-disulfide core (WFDC) domain, and is thus a member of the WFDC domain family

Long Name

Elastase Inhibitor, Transglutaminase Substrate, Serine Protease

Alternate Names

Elafin, ESI, PI3, SKALP, Trappin2

Gene Symbol

PI3

Additional Trappin-2/Elafin Products

Product Documents for Trappin-2/Elafin/Skalp Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Trappin-2/Elafin/Skalp Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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