TRBP Antibody (9E6C9)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16753

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16753-100ul
NBP3-16753-20ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 9E6C9

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all TRBP Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 200-300 of human TRBP (Q15633). RFIEIGSGTSKKLAKRNAAAKMLLRVHTVPLDARDGNEVEPDDDHFSIGVGSRLDGLRNRGPGCTWDSLRNSVGEKILSLRSCSLGSLGALGPACCRVLSE

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TRBP Antibody (9E6C9)

Western Blot: TRBP Antibody (9E6C9) [NBP3-16753]

Western Blot: TRBP Antibody (9E6C9) [NBP3-16753]

Western Blot: TRBP Antibody (9E6C9) [NBP3-16753] - Western blot analysis of extracts of various cell lines, using TRBP Rabbit mAb (NBP3-16753) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
Immunocytochemistry/ Immunofluorescence: TRBP Antibody (9E6C9) [NBP3-16753]

Immunocytochemistry/ Immunofluorescence: TRBP Antibody (9E6C9) [NBP3-16753]

Immunocytochemistry/Immunofluorescence: TRBP Antibody (9E6C9) [NBP3-16753] - Immunofluorescence analysis of U-2 OS cells using TRBP Rabbit mAb (NBP3-16753) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: TRBP Antibody (9E6C9) [NBP3-16753]

Immunohistochemistry-Paraffin: TRBP Antibody (9E6C9) [NBP3-16753]

Immunohistochemistry-Paraffin: TRBP Antibody (9E6C9) [NBP3-16753] - Immunohistochemistry of paraffin-embedded mouse pancreas using TRBP Rabbit mAb (NBP3-16753) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Applications for TRBP Antibody (9E6C9)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: TRBP

HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. The protein encoded by this gene binds between the bulge and the loop of the HIV-1 TAR RNA regulatory element and activates HIV-1 gene expression in synergy with the viral Tat protein. Alternative splicing results in multiple transcript variants encoding different isoforms. This gene also has a pseudogene.

Alternate Names

LOQS, RISC-loading complex subunit TARBP2, TAR (HIV) RNA binding protein 2, TAR (HIV) RNA-binding protein 2, TAR (HIV) RNA-binding protein TRBP1, TAR (HIV-1) RNA binding protein 2, TAR RNA binding protein 2, TAR RNA-binding protein 2, trans-activation responsive RNA-binding protein, Trans-activation-responsive RNA-binding protein, TRBP, TRBP1, TRBP2

Gene Symbol

TARBP2

Additional TRBP Products

Product Documents for TRBP Antibody (9E6C9)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for TRBP Antibody (9E6C9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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