TRBP Antibody (9E6C9)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16753
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 9E6C9
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for TRBP Antibody (9E6C9)
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 200-300 of human TRBP (Q15633). RFIEIGSGTSKKLAKRNAAAKMLLRVHTVPLDARDGNEVEPDDDHFSIGVGSRLDGLRNRGPGCTWDSLRNSVGEKILSLRSCSLGSLGALGPACCRVLSE
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for TRBP Antibody (9E6C9)
Western Blot: TRBP Antibody (9E6C9) [NBP3-16753]
Western Blot: TRBP Antibody (9E6C9) [NBP3-16753] - Western blot analysis of extracts of various cell lines, using TRBP Rabbit mAb (NBP3-16753) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.Immunocytochemistry/ Immunofluorescence: TRBP Antibody (9E6C9) [NBP3-16753]
Immunocytochemistry/Immunofluorescence: TRBP Antibody (9E6C9) [NBP3-16753] - Immunofluorescence analysis of U-2 OS cells using TRBP Rabbit mAb (NBP3-16753) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.Immunohistochemistry-Paraffin: TRBP Antibody (9E6C9) [NBP3-16753]
Immunohistochemistry-Paraffin: TRBP Antibody (9E6C9) [NBP3-16753] - Immunohistochemistry of paraffin-embedded mouse pancreas using TRBP Rabbit mAb (NBP3-16753) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.Applications for TRBP Antibody (9E6C9)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Immunohistochemistry
1:50 - 1:200
Immunohistochemistry-Paraffin
1:1000 - 1:2500
Western Blot
1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: TRBP
Alternate Names
LOQS, RISC-loading complex subunit TARBP2, TAR (HIV) RNA binding protein 2, TAR (HIV) RNA-binding protein 2, TAR (HIV) RNA-binding protein TRBP1, TAR (HIV-1) RNA binding protein 2, TAR RNA binding protein 2, TAR RNA-binding protein 2, trans-activation responsive RNA-binding protein, Trans-activation-responsive RNA-binding protein, TRBP, TRBP1, TRBP2
Gene Symbol
TARBP2
Additional TRBP Products
Product Documents for TRBP Antibody (9E6C9)
Product Specific Notices for TRBP Antibody (9E6C9)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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