TRIAD3 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-76592

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NBP1-76592
NBP1-76592-0.025mg
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all TRIAD3 Antibodies »

Product Specifications

Immunogen

Antibody was raised against a peptide corresponding to 15 amino acids near the amino-terminus of mouse TRIAD3A. The immunogen is located within amino acids 120 - 170 of TRIAD3A. Amino Acid Squence: TESDPLETQNQSSED

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

95 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for TRIAD3 Antibody - BSA Free

Western Blot: TRIAD3 AntibodyBSA Free [NBP1-76592]

Western Blot: TRIAD3 AntibodyBSA Free [NBP1-76592]

Western Blot: TRIAD3 Antibody [NBP1-76592] - Analysis of TRIAD3 in mouse heart tissue lysate with TRIAD3 antibody at (A) 1 and (B) 2 ug/mL.
Immunocytochemistry/ Immunofluorescence: TRIAD3 Antibody - BSA Free [NBP1-76592]

Immunocytochemistry/ Immunofluorescence: TRIAD3 Antibody - BSA Free [NBP1-76592]

Immunocytochemistry/Immunofluorescence: TRIAD3 Antibody [NBP1-76592] - Immunofluorescence of TRIAD3 in Mouse Heart cells with TRIAD3 antibody at 20 ug/mL.
Immunohistochemistry-Paraffin: TRIAD3 Antibody - BSA Free [NBP1-76592]

Immunohistochemistry-Paraffin: TRIAD3 Antibody - BSA Free [NBP1-76592]

Immunohistochemistry-Paraffin: TRIAD3 Antibody [NBP1-76592] - Mouse heart with TRIAD3 antibody at 10 ug/mL.
View 4 more images

Applications for TRIAD3 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

20 ug/ml

Immunohistochemistry

10 ug/ml

Immunohistochemistry-Paraffin

10 ug/mL

Western Blot

1-2 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TRIAD3

Activation of NF-kappaB as a result of Toll-like receptor (TLR) and IL-1 receptor signaling is a major component of innate immune responses. Signals from these receptors are relayed by a number of adapter molecules such as TRIF, TIRAP, and MyD88. Several regulatory mechanisms exist to control TLR signal transduction, including the inhibition of TLR expression and signaling by molecules such as ST2 and SIGIRR. Another mechanism is by the ubi-quitinization of selected TLRs by TRIAD3A, an E3 ubiquitin-protein ligase. TRIAD3A is a RING finger protein that can bind to TLR4 and TLR9, and to a lesser extent TLR3 and TLR5, catalyzing the ubiquitization of these molecules. Overexpression of TRIAD3A promoted the nearly complete degradation of TLR4 and TLR9; this reduction was reflected in the decreased signal-specific activation by ligands specific for these TLRs. Conversely, depletion of TRIAD3A resulted in enhanced TLR activation.

Alternate Names

EC 6.3.2.-, ring finger protein 216Ubiquitin-conjugating enzyme 7-interacting protein 1, Triad domain-containing protein 3, TRIAD3Zinc finger protein inhibiting NF-kappa-B, U7I1, UBCE7IP1ubiquitin conjugating enzyme 7 interacting protein 1, ZINE3 ubiquitin-protein ligase RNF216

Gene Symbol

RNF216

UniProt

AAP47174 (Human)

Additional TRIAD3 Products

Product Documents for TRIAD3 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for TRIAD3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.