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TRIB1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-20988

Novus Biologicals, part of Bio-Techne
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NBP1-20988

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

Product Specifications

Immunogen

Peptide with sequence C-TSDQIVPEYQEDSD corresponding to C-Terminus according to NP_079471.1 .

Epitope

C terminus

Reactivity Notes

Expected from sequence similarity: Mouse and Bovine

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for TRIB1 Antibody

Immunohistochemistry-Paraffin: TRIB1 Antibody [NBP1-20988]

Immunohistochemistry-Paraffin: TRIB1 Antibody [NBP1-20988]

Immunohistochemistry-Paraffin: TRIB1 Antibody [NBP1-20988] - (3.75ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for TRIB1 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

3.75 ug/ml

Peptide ELISA

Detection limit 1:8000

Western Blot

0.3 ug/ml
Application Notes
Western blot: Preliminary experiments gave an approx 35kDa band in Human Lymph Node and Peripheral Blood Mononucleocyte lysates after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 41.0kDa according to NP_079471.1. The 35kDa band was successfully blocked by incubation with the immunizing peptide.
IHC: Paraffin embedded Human Spleen.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: TRIB1

Interacts with MAPK kinases and regulates activation of MAP kinases. May not display kinase activity

Alternate Names

C8FWG-protein-coupled receptor induced protein, GIG-2, GIG2G-protein-coupled receptor-induced protein 2, G-protein-coupled receptor-induced gene 2 protein, SKIP1, TRB-1, TRB1phosphoprotein regulated by mitogenic pathways, tribbles homolog 1, tribbles homolog 1 (Drosophila), tribbles-like protein 1

Entrez Gene IDs

10221 (Human); 211770 (Mouse)

Gene Symbol

TRIB1

Additional TRIB1 Products

Product Documents for TRIB1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for TRIB1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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