TRIM Antibody (TRIM-04) [Janelia Fluor® 635]

Novus Biologicals, part of Bio-Techne | Catalog # NB500-485JF635

Novus Biologicals, part of Bio-Techne
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NB500-485JF635
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NB500-485
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Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, Flow (Intracellular), Flow Cytometry, Immunoprecipitation, Western Blot

Label

Janelia Fluor 635

Antibody Source

Monoclonal Mouse IgG2A Clone # TRIM-04

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all TRIM Antibodies »

Product Specifications

Immunogen

Recombinant fragment, corresponding to amino acids 29-186 of Human TRIM.

Specificity

The antibody TRIM-04 reacts with T cell receptor-interacting molecule (TRIM), a 30 kDa adaptor protein expressed by T cells.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Applications for TRIM Antibody (TRIM-04) [Janelia Fluor® 635]

Application
Recommended Usage

CyTOF-ready

Optimal dilutions of this antibody should be experimentally determined.

Flow (Intracellular)

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: TRIM

TRIM is a 30 kD disulfide-linked protein also known as T cell receptor interacting molecule. TRIM is a type III membrane protein containing a very short extracellular domain (8 amino acids). TRIM is highly expressed in T cells with some expression also observed in NK cells. TRIM is a signaling adaptor molecule that links extracellular and intracellular signaling. The TRIM protein is phosphorylated by Src kinases and associates with the PI3K regulatory subunit alpha after phosphorylation. TRIM has also been shown to associate with TCR alpha, beta, and zeta. The TRIM-4 monoclonal antibody recognizes human TRIM and has been shown to be useful for Western blotting, intracellular staining by flow cytometry, and immunofluorescence staining.

Long Name

T Cell Receptor Interacting Molecule

Alternate Names

TCRIM, TRAT1

Gene Symbol

TRAT1

Additional TRIM Products

Product Documents for TRIM Antibody (TRIM-04) [Janelia Fluor® 635]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for TRIM Antibody (TRIM-04) [Janelia Fluor® 635]



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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