Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1D11

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all Trim22 Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 61-406 of human TRIM22(NP_006065) produced in E.coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

56.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Trim22 Antibody (OTI1D11) - Azide and BSA Free

Western Blot: Trim22 Antibody (OTI1D11)Azide and BSA Free [NBP2-74608]

Western Blot: Trim22 Antibody (OTI1D11)Azide and BSA Free [NBP2-74608]

Western Blot: Trim22 Antibody (OTI1D11) - Azide and BSA Free [NBP2-74608] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRIM22.
Immunocytochemistry/ Immunofluorescence: Trim22 Antibody (OTI1D11) - Azide and BSA Free [NBP2-74608]

Immunocytochemistry/ Immunofluorescence: Trim22 Antibody (OTI1D11) - Azide and BSA Free [NBP2-74608]

Immunocytochemistry/Immunofluorescence: Trim22 Antibody (OTI1D11) - Azide and BSA Free [NBP2-74608] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY TRIM22.
Immunohistochemistry: Trim22 Antibody (OTI1D11) - Azide and BSA Free [NBP2-74608]

Immunohistochemistry: Trim22 Antibody (OTI1D11) - Azide and BSA Free [NBP2-74608]

Immunohistochemistry: Trim22 Antibody (OTI1D11) - Azide and BSA Free [NBP2-74608] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
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Applications for Trim22 Antibody (OTI1D11) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Western Blot

1:500

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Trim22

Trim22 is encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein localizes to the cytoplasm and its expression is induced by interferon. The protein down-regulates transcription from the HIV-1 LTR promoter region, suggesting that function of this protein may be to mediate interferon's antiviral effects. [provided by RefSeq]

Alternate Names

50 kDa-stimulated trans-acting factor, E3 ubiquitin-protein ligase TRIM22, EC 6.3.2, EC 6.3.2.-, GPSTAF50, RING finger protein 94, RNF94stimulated trans-acting factor (50 kDa), Staf-50, STAF50staf-50, tripartite binding motif 22, tripartite motif containing 22, tripartite motif protein TRIM22, tripartite motif-containing 22, Tripartite motif-containing protein 22

Gene Symbol

TRIM22

Additional Trim22 Products

Product Documents for Trim22 Antibody (OTI1D11) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Trim22 Antibody (OTI1D11) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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